Overexpression, purification and characterization of two pyrimidine kinases involved in the biosynthesis of thiamin: 4-amino-5-hydroxymethyl-2-methylpyrimidine kinase and 4-amino-5-hydroxymethyl-2-methylpyrimidine phosphate kinase

The overexpression, purification and characterization of 4-amino-5-hydroxymethyl-2-methylpyrimidine kinase (HMP kinase) and 4-amino-5-hydroxymethyl-2-methylpyrimidine monophosphate kinase (HMP-P kinase) are described. Surprisingly HMP-P kinase also shows HMP kinase activity. These enzymes are useful...

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Published inTetrahedron Vol. 54; no. 52; pp. 15983 - 15991
Main Authors Reddick, Jason J, Kinsland, Cynthia, Nicewonger, Robb, Christian, Todd, Downs, Diana M, Winkler, Malcolm E, Begley, Tadhg P
Format Journal Article
LanguageEnglish
Published OXFORD Elsevier Ltd 24.12.1998
Elsevier
Subjects
biosynthesis
Chemistry
Chemistry, Organic
Enzymes
phosphorylation
Physical Sciences
Science & Technology
Enzymes
phosphorylation
biosynthesis
PYROPHOSPHOKINASE
GENE
SALMONELLA-TYPHIMURIUM
enzymes
PYROPHOSPHORYLASE
OPERON
SACCHAROMYCES-CEREVISIAE
HYDROXYETHYLTHIAZOLE KINASE
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Summary:The overexpression, purification and characterization of 4-amino-5-hydroxymethyl-2-methylpyrimidine kinase (HMP kinase) and 4-amino-5-hydroxymethyl-2-methylpyrimidine monophosphate kinase (HMP-P kinase) are described. Surprisingly HMP-P kinase also shows HMP kinase activity. These enzymes are useful reagents for the preparation of intermediates on the thiamin biosynthetic pathway. Graphic
ISSN:0040-4020
1464-5416
DOI:10.1016/S0040-4020(98)01006-0