Microplastics from agricultural mulch films: Biodegradation and ecotoxicity in freshwater systems

• Published in: The Science of the total environment Vol. 912; p. 169287
• Main Authors: Ferreira-Filipe, Diogo A., Paço, Ana, Pinho, Bruna, Silva, Rafael, Silva, Sara A.M., Jesus, Fátima, Pereira, Joana L., Duarte, Armando C., Rocha-Santos, Teresa A.P., Patrício-Silva, Ana L.
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 20.02.2024
• Subjects: Bio-based plastics; Biomarkers; Chironomids; fungi biodegradation; Microplastic pollution; Biomarkers; Microplastic pollution; Bio-based plastics; Chironomids; fungi biodegradation
• ISSN: 0048-9697; 1879-1026
• DOI: 10.1016/j.scitotenv.2023.169287

The application of bio-based biodegradable mulch films in agriculture has raised environmental concerns regarding their potential impacts on adjacent freshwater ecosystems. This study investigated the biodegradation of microplastics derived from a bio-based biodegradable mulch (bio-MPs) and its acute and chronic ecotoxicity considering relevant scenarios (up to 200 and 250 mg/kg of sediment, using pristine and/or UV-aged particles), using the fungus Penicillium brevicompactum and the dipteran Chironomus riparius as model organisms, respectively, due to their ecological relevance in freshwater environments. Fourier-transform infrared spectroscopy analysis suggested changes in the fungus's carbohydrate reserves and bio-MP degradation through the appearance of low molecular weight esters throughout a 28 day biodegradation test. In a short-term exposure (48 h), C. riparius larvae exposed to pristine or UV-aged bio-MPs had up to 2 particles in their gut. Exposure to pristine bio-MPs decreased larval aerobic metabolism (<20 %) and increased neurotransmission (>15 %), whereas exposure to UV-aged bio-MPs activated larval aerobic metabolism (>20 %) and increased antioxidant defences (catalase activity by >30 % and glutathione-s-transferase by >20 %) and neurotransmission (>30 %). Longer-term (28-d) exposure to UV-aged bio-MPs did not affect larval survival and growth nor the dipteran's emergence but increased male numbers (>30 %) at higher concentrations. This study suggests that the selected agricultural bio-based mulch film is prone to biodegradation by a naturally occurring fungus. However, there is a potential for endocrine disruption in the case of prolonged exposures to UV-aged microplastics. This study emphasises the importance of further research to elucidate the potential ecological effects of these plastic products, to ensure effective management practices, and to establish new regulations governing their use. [Display omitted] •Bio-based biodegradable mulch (bio-MPs) revealed biodegradability by P. brevicompactum.•Pristine bio-MPs decreased C. riparius aerobic metabolism and increased neurotransmission.•UV-aged bio-MPs increased C. riparius metabolism, antioxidant defences and neurotransmission.•UV-aged bio-MPs increased C. riparius male numbers at the highest concentrations.