Aqueous-based microcapsules are detected primarily in gut-associated dendritic cells after oral inoculation of mice

• Published in: Vaccine Vol. 15; no. 17; pp. 1959 - 1962
• Main Authors: Lomotan, Edwin A., Brown, Kurt A., Speaker, Tully J., Offita, Paul A.
• Format: Journal Article
• Language: English
• Published: Oxford Elsevier Ltd 01.12.1997; Elsevier
• Subjects: Administration, Oral; Alginates - pharmacokinetics; Animals; Applied microbiology; Biological and medical sciences; Capsules; dendritic cells; Dendritic Cells - metabolism; Female; Fundamental and applied biological sciences. Psychology; Glucuronic Acid; gut-associated lymphoid tissue; Hexuronic Acids; Intestinal Mucosa - metabolism; Intestines - cytology; Intestines - immunology; Lymphoid Tissue - cytology; Lymphoid Tissue - immunology; Lymphoid Tissue - metabolism; Mice; Microbiology; microcapsules; Rhodamines - pharmacokinetics; Spermine - pharmacokinetics; Spleen - metabolism; Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects); Water; gut-associated lymphoid tissue; microcapsules; dendritic cells; Antigen presentation; Immunostimulation; Controlled release form; Rodentia; Microcapsule; Vaccine; Alginates; Property formulation relationship; Vertebrata; Mammalia; Spermine; Mouse; Mechanism of action
• ISSN: 0264-410X; 1873-2518
• DOI: 10.1016/S0264-410X(97)00108-4

We previously found that aqueous-based microencapsulation enhanced virus-specific humoral immune responses after oral inoculation of mice. However, the mechanism by which microencapsulation enhances immunogenicity remains unclear. We found that spermine-alginate microcapsules were detected primarily in gut-associated dendritic cells (i.e. CD11c/CD18 +, Ia +, CD11b −, CD45R −) after oral inoculation of adult mice. Microencapsulation may enhance immunogenicity by involving antigen presenting cells which are more efficient than those recruited during natural infection.