Telomere-to-Telomere Genome Assembly of Tibetan Medicinal Mushroom Ganoderma leucocontextum and the First Copia Centromeric Retrotransposon in Macro-Fungi Genome

• Published in: Journal of fungi (Basel) Vol. 10; no. 1; p. 15
• Main Authors: Wang, Miao, Meng, Guoliang, Yang, Ying, Wang, Xiaofang, Xie, Rong, Dong, Caihong
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 01.01.2024
• Subjects: Cell cycle; centromere; Centromeres; Chromosomes; Copia; Fungi; Ganoderma; Gene mapping; Genomes; Genomic analysis; Mushrooms; Nucleotide sequence; Sequence analysis; Software; telomere-to-telomere; Telomeres; Tibet; United States > US; China; Copia; centromere; telomere-to-telomere; Ganoderma
• ISSN: 2309-608X; 2309-608X
• DOI: 10.3390/jof10010015

A complete telomere-to-telomere (T2T) genome has been a longstanding goal in the field of genomic research. By integrating high-coverage and precise long-read sequencing data using multiple assembly strategies, we present here the first T2T gap-free genome assembly of Ganoderma leucocontextum strain GL72, a Tibetan medicinal mushroom. The T2T genome, with a size of 46.69 Mb, consists 13 complete nuclear chromosomes and typical telomeric repeats (CCCTAA)n were detected at both ends of 13 chromosomes. The high mapping rate, uniform genome coverage, a complete BUSCOs of 99.7%, and base accuracy exceeding 99.999% indicate that this assembly represents the highest level of completeness and quality. Regions characterized by distinct structural attributes, including highest Hi-C interaction intensity, high repeat content, decreased gene density, low GC content, and minimal or no transcription levels across all chromosomes may represent potential centromeres. Sequence analysis revealed the first Copia centromeric retrotransposon in macro-fungi genome. Phylogenomic analysis identified that G. leucocontextum and G. tsugae diverged from the other Ganoderma species approximately 9.8–17.9 MYA. The prediction of secondary metabolic clusters confirmed the capability of this fungus to produce a substantial quantity of metabolites. This T2T gap-free genome will contribute to the genomic ‘dark matter’ elucidation and server as a great reference for genetics, genomics, and evolutionary studies of G. leucocontextum.