Effects of exogenous cholecystokinin and gastrin on the secretion of trypsin and chymotrypsin from yellowtail ( Seriola quinqueradiata) isolated pyloric caeca

The humoral control of secretion of the proteolytic enzymes trypsin and chymotrypsin was studied in yellowtail ( Seriola quinqueradiata). In vitro trials were performed to investigate the effects of cholecystokinin (CCK) and two commercially available gastrin peptides. Isolated preparations of pylor...

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Published inComparative biochemistry and physiology. Part A, Molecular & integrative physiology Vol. 146; no. 1; pp. 124 - 130
Main Authors Kofuji, Patricia Y.M., Murashita, Koji, Hosokawa, Hidetsuyo, Masumoto, Toshiro
Format Journal Article
LanguageEnglish
Published United States Elsevier Inc 2007
Subjects
Animals
Cholecystokinin
Cholecystokinin - pharmacology
Chymotrypsin
Chymotrypsin - secretion
Gastrin
Gastrins - pharmacology
In vitro assays
Pancreas
Pancreas - drug effects
Pancreas - secretion
Perciformes - physiology
Proteolytic enzyme secretion
Pyloric caeca, trypsin
Pylorus - drug effects
Pylorus - secretion
Temperature
Trypsin - secretion
Yellowtail ( Seriola quinqueradiata)
Proteolytic enzyme secretion
Chymotrypsin
Temperature
Gastrin
Pyloric caeca, trypsin
Yellowtail ( Seriola quinqueradiata)
Cholecystokinin
Pancreas
In vitro assays
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Summary:The humoral control of secretion of the proteolytic enzymes trypsin and chymotrypsin was studied in yellowtail ( Seriola quinqueradiata). In vitro trials were performed to investigate the effects of cholecystokinin (CCK) and two commercially available gastrin peptides. Isolated preparations of pyloric caeca/pancreas release trypsin and chymotrypsin when incubated with cholecystokinin (CCK) at 10 μM and gastrin I (G1) at 50 μM after 15 min of incubation. On the other hand, G1 at 10 μM and gastrin-related peptide (G2) did not enhance trypsin and chymotrypsin secretion. The studies concerning the CCK effects at different incubation temperatures have shown that trypsin and chymotrypsin secretion at 25 °C was stimulated by CCK after 15 min, while at 10, 15 and 20 °C the stimulatory effects of CCK were observed only after 30 min of incubation. The CCK effects were increased at higher incubation temperatures and longer incubation periods.
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ISSN:1095-6433
1531-4332
DOI:10.1016/j.cbpa.2006.09.017