A major role for CD62P/CD15s interaction in leukocyte margination during hemodialysis

• Published in: Kidney international Vol. 48; no. 1; pp. 93 - 102
• Main Authors: Stuard, Stefano, Carreno, Marie-Paule, Poignet, Jean-Louis, Albertazzi, Alberto, Haeffner-Cavaillon, Nicole
• Format: Journal Article
• Language: English
• Published: New York, NY Elsevier Inc 01.07.1995; Nature Publishing
• Subjects: Acrylic Resins; Adult; Aged; Anesthesia. Intensive care medicine. Transfusions. Cell therapy and gene therapy; Biological and medical sciences; CD11 Antigens - immunology; Cellulose - analogs & derivatives; Emergency and intensive care: renal failure. Dialysis management; Flow Cytometry; Humans; Intensive care medicine; Leukocyte Count; Leukocytes - immunology; Lewis X Antigen - immunology; Medical sciences; Membranes, Artificial; Middle Aged; Monocytes - immunology; Neutrophils - immunology; P-Selectin - immunology; Platelet Activation - physiology; Renal Dialysis - methods; Human; Monocyte; Leukocyte; Hemodialysis; Cell adhesion molecule; Granulocyte; Artificial membrane; Gene expression; Adhesion; Extrarenal dialysis; Integrin; Biocompatibility; Biomaterial; Neutrophil
• ISSN: 0085-2538; 1523-1755
• DOI: 10.1038/ki.1995.272

A major role of CD62P/CD15s interaction in leukocyte margination during hemodialysis. We investigated expression of several antigens on neutrophils and monocytes, involved in cell adhesion, from patients hemodialyzed with cellulosic and polyacrylonitrile membranes. Among the antigens tested only the expression of CD15s and CD11b was significantly increased on neutrophils and monocytes in patients dialyzed with cellulosic membranes. No changes occurred with polyacrylonitrile membranes. Leukocyte counts from patients dialyzed with cuprophane membranes decreased at the same time as expression of cellular CD15s increased, resulting in a significant negative correlation at all time points tested. No correlation was found between the drop of monocytes and their expression of CD11b. When CD 15s expression increased on neutrophils and monocytes, we observed a concomitant increase of CD62P, a specific selectin of activated platelets. When whole blood cells were incubated with complement activated serum both antigens increased but not when cells were incubated with hrC5a. We also observed that CD61, a platelet phenotypic antigen, was present on leukocytes incubated with complement activated serum. At the time when platelet-leukocyte coaggregates decreased, CD62P expression remained stable on leukocytes, suggesting that both neutrophils and monocytes are able to trap either CD62P shed by activated platelets or soluble CD62P present in normal human serum. The present study documents a major role of P-selectin (CD62P)/sialyl-Lewis x (CD 15s) interaction in the transient leukocyte margination during hemodialysis.