Molecular mapping and physical location of major gene conferring seedling resistance to Septoria tritici blotch in wheat

• Published in: Molecular breeding Vol. 24; no. 2; pp. 153 - 164
• Main Authors: Raman, R, Milgate, A. W, Imtiaz, M, Tan, M.-K, Raman, H, Lisle, C, Coombes, N, Martin, P
• Format: Journal Article
• Language: English
• Published: Dordrecht Dordrecht : Springer Netherlands 01.09.2009; Springer Netherlands; Springer Nature B.V
• Subjects: Biomedical and Life Sciences; Biotechnology; Chara; Chromosomes; Conditioning; Foliar diseases; Gene mapping; Heredity; Imperfect state; Life Sciences; Mapping; Marker-assisted selection; Markers; Molecular biology; Mycosphaerella graminicola; Pathogens; Phenotypic variations; Physical mapping; Plant biology; Plant breeding; Plant Genetics and Genomics; Plant Pathology; Plant Physiology; Plant Sciences; Populations; Seedlings; Septoria tritici; Septoria tritici blotch; Triticum aestivum; Wheat; Molecular mapping; Disease resistance; Physical mapping; Marker validation
• ISSN: 1380-3743; 1572-9788
• DOI: 10.1007/s11032-009-9280-0

Septoria tritici blotch (STB) caused by Mycosphaerella graminicola (anamorph: Septoria tritici), is one of the most important foliar diseases of wheat. We assessed three doubled-haploid (DH) populations derived from Chara (STB-susceptible)/WW2449 (STB-resistant), Whistler (STB-susceptible)/WW1842 (STB-resistant) and Krichauff (STB susceptible)/WW2451 (STB-resistant) for resistance to a single-pycnidium isolate 79.2.1A of M. graminicola at the seedling stage. STB resistance in each of the three DH populations was conditioned by a single major gene designated as StbWW2449, StbWW1842 and StbWW2451. Linkage analyses and physical mapping indicated that the StbWW loci were located on the short arm of chromosome 1B (IBS). Four simple sequence repeat (SSR) markers linked with STB resistance: Xwmc230, Xbarc119b, Xksum045 and Xbarc008 were located to the distal bin of 1BS.sat1BS-4 (FL: 0.52-1.00) in the 1BS physical map. Xwmc230, Xbarc119b and Xksum045 markers, mapped within 7 cM from StbWW were validated for their linkage and predicted the STB resistance with over 94% accuracy in the 79 advanced breeding lines having WW2449 as one of the parents. The marker interval Xwmc230/Xksum045-Xbarc119b also explained up to 38% of the phenotypic variance at the adult plant stage in all three DH mapping populations. These results have proven that SSR markers are useful in monitoring STB resistance both at seedling and adult plant stages and hence are suitable for routine marker-assisted selection in the wheat breeding programs.