Protection against tetanus toxin in mice nasally immunized with recombinant Lactococcus lactis expressing tetanus toxin fragment C

• Published in: Vaccine Vol. 15; no. 6; pp. 616 - 619
• Main Authors: Norton, P.M., Wells, J.M., Brown, H.W.G., Macpherson, A.M., Le Page, R.W.F.
• Format: Journal Article
• Language: English
• Published: Oxford Elsevier Ltd 01.04.1997; Elsevier
• Subjects: Administration, Intranasal; Animals; Bacteriology; Biological and medical sciences; Clostridium tetani; Fundamental and applied biological sciences. Psychology; Genetic Vectors; Immunity, Mucosal; intranasal immunization; Lactococcus lactis; Mice; Mice, Inbred C57BL; Microbiology; mucosal immunization; Tetanus Antitoxin - blood; tetanus toxin; Tetanus Toxin - immunology; Tetanus Toxoid - immunology; vaccine; Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies; Vaccines, Synthetic - immunology; mucosal immunization; tetanus toxin; vaccine; lactococcus lactis; intranasal immunization; Recombinant microorganism; Lactococcus lactis; Mucosa; Lung; Rodentia; Vaccine; Intranasal administration; Infection; Streptococcaceae; Toxin; Vertebrata; Local immunity; Mammalia; Immunogenicity; Mouse; Nose; Bacteriosis; Bacteria; Micrococcales; Tetanus; Humoral immunity
• ISSN: 0264-410X; 1873-2518
• DOI: 10.1016/S0264-410X(96)00241-1

Mice inoculated intranasally (i.n.) with a recombinant strain of live Lactococcus lactis expressing tetanus toxin fragment C (TTFC), produced both serum and secretory antibodies to TTFC. Killed bacteria which had accumulated TTFC intracellularly in vitro also elicited protective serum antibody responses. There was no requirement for either colonization or invasion of the mucosa. In addition secretory antibody responses in the lung and nasal tissues were elicited after i.n. inoculation in the presence of an adjuvant.