Determination of rofecoxib in human plasma and breast milk by high-performance liquid chromatographic assay

• Published in: Journal of chromatography. B, Analytical technologies in the biomedical and life sciences Vol. 807; no. 2; pp. 217 - 221
• Main Authors: Zhang, Mei, Moore, Grant A, Gardiner, Sharon J, Begg, Evan J
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 05.08.2004; Elsevier Science
• Subjects: Analysis; Analytical, structural and metabolic biochemistry; Biological and medical sciences; Chromatography, High Pressure Liquid - methods; Cyclooxygenase Inhibitors - analysis; Cyclooxygenase Inhibitors - blood; Fundamental and applied biological sciences. Psychology; General pharmacology; Humans; Lactones - analysis; Lactones - blood; Medical sciences; Milk, Human - chemistry; Pharmacology. Drug treatments; Reproducibility of Results; Rofecoxib; Sulfones - analysis; Sulfones - blood; Rofecoxib; Human; Prostaglandin-endoperoxide synthase; Solid phase extraction; Biological fluid; Enzyme; Enzyme inhibitor; HPLC chromatography; Cyclooxygenase 2 inhibitor; Blood plasma; Non steroidal antiinflammatory agent; Ultraviolet detector; Investigation method; Oxidoreductases; Quantitative analysis; Breast milk
• ISSN: 1570-0232; 1873-376X
• DOI: 10.1016/j.jchromb.2004.04.011

A rapid and simple HPLC assay was developed for the determination of rofecoxib in human plasma and breast milk. After solid-phase extraction, rofecoxib was resolved on a C18 column and detected by UV detection at 272 nm. Standard curves were linear over the concentration range 10–2000 μg/L ( r 2>0.99). Intra- and inter-day coefficients of variation for both matrices were <10% and the limit of quantification was around 10 μg/L.