Molecular studies of the intestinal mucosal barrier physiopathology using cocultures of epithelial and immune cells: a technical update

• Published in: Microbes and Infection Vol. 2; no. 9; pp. 1119 - 1124
• Main Authors: Kernéis, Sophie, Caliot, Elise, Stubbe, Hiltrud, Bogdanova, Anna, Kraehenbuhl, Jean-Pierre, Pringault, Eric
• Format: Book Review Journal Article
• Language: English
• Published: Lausanne Elsevier SAS 01.07.2000; Amsterdam Elsevier; Paris
• Subjects: Animals; Bacterial Proteins - analysis; Bacteriology; Biological and medical sciences; Caco-2 Cells - immunology; Cell Differentiation; coculture; Coculture Techniques - methods; Diverse techniques; Enzyme-Linked Immunosorbent Assay; Epithelial Cells - immunology; Fundamental and applied biological sciences. Psychology; gut; Humans; M cells; Mice; Mice, Inbred BALB C; Microbiology; Microscopy, Electron; Molecular and cellular biology; mucosal immunity; Pathogenicity, virulence, toxins, bacteriocins, pyrogens, host-bacteria relations, miscellaneous strains; Peyer's Patches - immunology; Peyer′s patches; Phagocytosis; Ribosomal Proteins - analysis; Vibrio cholerae; coculture; M cells; Peyer′s patches; gut; mucosal immunity; Human; Translocation; Peyer patch; Digestive system; Mucosa; Gut; Host agent relation; Review; Epithelium; Immunity; Mixed cell culture; Cell line; Bacteria; Enterocyte; Models; Colon; Technique; Lymphocyte
• ISSN: 1286-4579; 1769-714X
• DOI: 10.1016/S1286-4579(00)01266-1

Peyer′s patch lymphocytes cocultured with Caco-2 cells trigger the phenotypic conversion of enterocytes into cells that express morphological and functional M-cell properties. We report a technical update for setting up this model, which will enable the study of M-cell biology, the identification by biochemical approaches of molecules involved in the interaction of microorganisms with M cells, and the development of vectors that would efficiently target the mucosal immune system.