Proline-glutamate interactions in the CNS

• Published in: Progress in neuro-psychopharmacology & biological psychiatry Vol. 21; no. 1; pp. 141 - 152
• Main Authors: Ortiz, Jose G., Cordero, Mayra L., Rosado, Arelys
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier Inc 1997; Elsevier
• Subjects: Animals; AP-7; Biological and medical sciences; Central nervous system; Central Nervous System - drug effects; Central neurotransmission. Neuromudulation. Pathways and receptors; CGS-19755; CPP; Drug Interactions; Fundamental and applied biological sciences. Psychology; glutamate; Glutamic Acid - pharmacology; HA-966; Male; Mice; Mice, Inbred C57BL; NMDA; Proline - pharmacology; Proline binding; Rats; Rats, Wistar; Vertebrates: nervous system and sense organs; cis-4-(phosphonomethyl) piperidine-2-carboxylic acid; L-glutamic acid; CPP; Proline binding; HA-966; R(+)-3-amino-l-hydroxy-2-pyrrolidone; AP-7; CGS-19755; (±)-3-(2-carboxypiperazin-4yl)-propyl-1-phosphonic acid; (dizocilpine), (5s, 10s)-(+)-5-methyl-10, 11-dihidro-5H-dibenzo[a,d]cyclohepten-5, 10-imine; 2-amino-7-phosphonoheptanoic acid; L-proline; NMDA; N-methyl-D-aspartate; glutamate; Rat; Rodentia; Central nervous system; Proline; Binding site; Glutamate; In vitro; Vertebrata; Biological fixation; Synapse; Mammalia; Mouse; Animal; Excitatory aminoacid; Brain (vertebrata)
• ISSN: 0278-5846; 1878-4216
• DOI: 10.1016/S0278-5846(96)00164-9

1. 1. Crude synaptosomes (P 2) and synaptosomal membranes were prepared from normal C57/B110 mouse brains and Wistar rats respectively. 2. 2. [ 3H]Pro binding to mouse brain synaptic membranes was examined in the presence of competitive NMDA antagonist, MK-801, or HA-966. Conversely, the effects of l-proline on [ 3H]MK-801 binding were also probed. The effects of l-proline on glutamatemedicated [Ca +2], levels were tested. 3. 3. The authors could not detect any effect of proline on glutamate-mediated [CA +2] i levels using FURA-2 in synaptosomes or neuroblastoma cells. 4. 4. NMDA competitive antagonists, AP-7, CPP, and CGS 19755 inhibit [ 3H]Pro binding to mouse brain synaptic membranes. 5. 5. MK-801, a NMDA channel blocker, also inhibits [ 3]Pro binding, but 200 mM praline is incapable of inhibiting [ 3H] MK-801 binding. 6. 6. HA-966, a glycine site partial agonist inhibits [ 3]Pro binding. Praline has modest effects on [ 3H]glycine binding.