Analysis of Altered Micro RNA Expression Profiles in Focal Cortical Dysplasia IIB

• Published in: Journal of child neurology Vol. 31; no. 5; p. 613
• Main Authors: Li, Lin, Liu, Chang-Qing, Li, Tian-Fu, Guan, Yu-Guang, Zhou, Jian, Qi, Xue-Ling, Yang, Yu-Tao, Deng, Jia-Hui, Xu, Zhi-Qing David, Luan, Guo-Ming
• Format: Journal Article
• Language: English
• Published: United States 01.04.2016
• Subjects: Biological Ontologies; Brain - diagnostic imaging; Child; Child, Preschool; Computational Biology; Epilepsy - drug therapy; Epilepsy - genetics; Epilepsy - metabolism; Female; Gene Expression Profiling; Gene Expression Regulation, Developmental - genetics; Hippocampus - metabolism; Humans; Image Processing, Computer-Assisted; Magnetic Resonance Imaging; Male; Malformations of Cortical Development, Group I - drug therapy; Malformations of Cortical Development, Group I - genetics; Malformations of Cortical Development, Group I - metabolism; MicroRNAs - genetics; MicroRNAs - metabolism; Oligonucleotide Array Sequence Analysis; Hippo signaling pathway; micro RNA; focal cortical dysplasia type IIB
• ISSN: 1708-8283
• DOI: 10.1177/0883073815609148

Focal cortical dysplasia type IIB is a commonly encountered subtype of developmental malformation of the cerebral cortex and is often associated with pharmacoresistant epilepsy. In this study, to investigate the molecular etiology of focal cortical dysplasia type IIB, the authors performed micro ribonucleic acid (RNA) microarray on surgical specimens from 5 children (2 female and 3 male, mean age was 73.4 months, range 50-112 months) diagnosed of focal cortical dysplasia type IIB and matched normal tissue adjacent to the lesion. In all, 24 micro RNAs were differentially expressed in focal cortical dysplasia type IIB, and the microarray results were validated using quantitative real-time polymerase chain reaction (PCR). Then the putative target genes of the differentially expressed micro RNAs were identified by bioinformatics analysis. Moreover, biological significance of the target genes was evaluated by investigating the pathways in which the genes were enriched, and the Hippo signaling pathway was proposed to be highly related with the pathogenesis of focal cortical dysplasia type IIB.