Determination of derivatized l-alanosine in plasma by liquid chromatography-tandem mass spectrometry

• Published in: Journal of chromatography. B, Analytical technologies in the biomedical and life sciences Vol. 803; no. 2; pp. 311 - 315
• Main Authors: Gantverg, Alex, Elliott, Gary, Pineault, Jean, Demers, Roger
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 25.04.2004; Elsevier Science
• Subjects: Alanine - analogs & derivatives; Alanine - blood; Alanosine; Analysis; Analytical, structural and metabolic biochemistry; Antibiotics, Antineoplastic - blood; Antineoplastic Agents - blood; Biological and medical sciences; Chromatography, Ion Exchange - methods; Derivatization, LC; Fundamental and applied biological sciences. Psychology; General pharmacology; Mass Spectrometry - methods; Medical sciences; Pharmacology. Drug treatments; Sensitivity and Specificity; Alanosine; Derivatization, LC; Human; Solid phase extraction; Biological fluid; Cytotoxicity; Liquid chromatography; Malignant tumor; Chemical ionization; Blood plasma; Antibiotic; Sensitivity; Chlorides; Mass spectrometry MS/MS; Quantitative analysis
• ISSN: 1570-0232; 1873-376X
• DOI: 10.1016/j.jchromb.2004.01.003

A sensitive method was developed for quantitation of the cytotoxic antibiotic l-alanosine in human plasma. Alanosine was extracted from plasma by anion-exchange solid phase extraction, derivatized with dansyl chloride and analyzed by liquid chromatography-tandem mass spectrometry using atmospheric pressure chemical ionization in negative mode. Dansylation led to 50-fold improvement of method sensitivity over non-dansylated alanosine with a resulting 20 ng/ml limit of alanosine quantitation in plasma being achieved. The method was validated and applied for clinical studies of alanosine administered to cancer patients.