Genomic clones of the human liver-type phosphofructokinase

• Published in: Biochemical and biophysical research communications Vol. 141; no. 1; pp. 374 - 380
• Main Authors: Levanon, D., Danciger, E., Dafni, N., Groner, Y.
• Format: Journal Article
• Language: English
• Published: San Diego, CA Elsevier Inc 26.11.1986; Elsevier
• Subjects: Animals; Applied sciences; Chromosomes, Human, Pair 21; Cloning, Molecular; Exact sciences and technology; Genes; Humans; Hybrid Cells - physiology; Liver - enzymology; Mice; Other techniques and industries; Phosphofructokinase-1 - genetics; Protein Biosynthesis; RNA, Messenger - genetics
• ISSN: 0006-291X; 1090-2104
• DOI: 10.1016/S0006-291X(86)80379-5

Genomic clones of human liver phosphofructokinase (PFK) were isolated by screening a gene bank enriched for chromosome 21 sequences with two synthetic oligonucleotide probes designed from peptide sequences of purified human liver PFK. A 3.3 Kb fragment derived from the genomic clones was sub-cloned and designated pG-PFKL 3.3. It hybridized with a 3.5 Kb mRNA on Northern blots and was able to enrich selectively for liver PFK mRNA by hybrid-selection. These results demonstrated that the isolated clones contain sequences homologous to human PFKL mRNA. When hybridized to genomic DNA blots pG-PFKL 3.3 reacted with the same 3.3 Kb BamHI fragment in both human DNA and DNA of the mouse/human hybrid line WA17 which contains human chromosome 21 as the only human chromosome. These data confirm the assignment of the PFKL gene to chromosome 21.