Bacterial community and genome analysis of cytoplasmic incompatibility-inducing Wolbachia in American serpentine leafminer, Liriomyza trifolii
, an agricultural pest, is occasionally infected by A strain present in is associated with cytoplasmic incompatibility (CI) effects, leading to the death of embryos resulting from incompatible crosses between antibiotic-treated or naturally -free strain females and -infected males. In this study, hi...
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Published in | Frontiers in microbiology Vol. 15; p. 1304401 |
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Main Authors | , , , |
Format | Journal Article |
Language | English |
Published |
Switzerland
Frontiers Media S.A
06.02.2024
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Subjects | |
Online Access | Get full text |
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Summary: | , an agricultural pest, is occasionally infected by
A
strain present in
is associated with cytoplasmic incompatibility (CI) effects, leading to the death of embryos resulting from incompatible crosses between antibiotic-treated or naturally
-free strain females and
-infected males. In this study, high-throughput sequencing of hypervariable rRNA genes was employed to characterize the bacterial community in
-infected
without antibiotic treatment. The analysis revealed that
dominates the bacterial community in
, with minor presence of
,
, and
. To elucidate the genetic basis of the CI phenotype, metagenomic sequencing was also conducted to assemble the genome of the
strain. The draft-genome of the
strain
Ltri was 1.35 Mbp with 34% GC content and contained 1,487 predicted genes. Notably, within the
Ltri genome, there are three distinct types of cytoplasmic incompatibility factor (
) genes: Type I, Type III, and Type V
. These genes are likely responsible for inducing the strong cytoplasmic incompatibility observed in
. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 Reviewed by: Sarah Bordenstein, The Pennsylvania State University (PSU), United States Mathieu Sicard, Université Montpellier 2, France Edited by: Didier Bouchon, University of Poitiers, France |
ISSN: | 1664-302X 1664-302X |
DOI: | 10.3389/fmicb.2024.1304401 |