Bacterial community and genome analysis of cytoplasmic incompatibility-inducing Wolbachia in American serpentine leafminer, Liriomyza trifolii

• Published in: Frontiers in microbiology Vol. 15; p. 1304401
• Main Authors: Pramono, Ajeng K, Hidayanti, Ardhiani K, Tagami, Yohsuke, Ando, Hiroki
• Format: Journal Article
• Language: English
• Published: Switzerland Frontiers Media S.A 06.02.2024
• Subjects: CIF; cytoplasmic incompatibility factor; Liriomyza trifolii; Microbiology; phage WO; Wolbachia; cytoplasmic incompatibility factor; CIF; phage WO; Wolbachia; Liriomyza trifolii
• ISSN: 1664-302X; 1664-302X
• DOI: 10.3389/fmicb.2024.1304401

, an agricultural pest, is occasionally infected by A strain present in is associated with cytoplasmic incompatibility (CI) effects, leading to the death of embryos resulting from incompatible crosses between antibiotic-treated or naturally -free strain females and -infected males. In this study, high-throughput sequencing of hypervariable rRNA genes was employed to characterize the bacterial community in -infected without antibiotic treatment. The analysis revealed that dominates the bacterial community in , with minor presence of , , and . To elucidate the genetic basis of the CI phenotype, metagenomic sequencing was also conducted to assemble the genome of the strain. The draft-genome of the strain Ltri was 1.35 Mbp with 34% GC content and contained 1,487 predicted genes. Notably, within the Ltri genome, there are three distinct types of cytoplasmic incompatibility factor ( ) genes: Type I, Type III, and Type V . These genes are likely responsible for inducing the strong cytoplasmic incompatibility observed in .