Incorporation of radioiodinated IPPA and BMIPP fatty acid analogues into complex lipids from isolated rat hearts

• Published in: International journal of radiation applications and instrumentation. Part B, Nuclear medicine and biology Vol. 19; no. 3; pp. 283 - 288
• Main Authors: Kropp, J., Ambrose, K.R., Knapp, F.F., Nissen, H.P., Biersack, H.J.
• Format: Journal Article
• Language: English
• Published: Oxford Elsevier Ltd 01.04.1992; Pergamon
• Subjects: Animals; Biological and medical sciences; Cattle; Chromatography, High Pressure Liquid; Fatty Acids - chemistry; Fatty Acids - metabolism; Female; Fundamental and applied biological sciences. Psychology; In Vitro Techniques; Iodine Radioisotopes; Iodobenzenes - metabolism; Isotope Labeling; Lipid Metabolism; Lipids - chemistry; Lipids. Glycolipids; Metabolisms and neurohumoral controls; Myocardium - chemistry; Myocardium - metabolism; Perfusion; Rats; Rats, Inbred F344; Vertebrates: anatomy and physiology, studies on body, several organs or systems; Heart; Rat; Rodentia; Phospholipid; Lipids; Radioactive tracers; Metabolism; Fatty acids; Isolated organ; Vertebrata; Mammalia; Animal; Myocardium; Circulatory system; Technique
• ISSN: 0883-2897
• DOI: 10.1016/0883-2897(92)90112-C

Heart lipids were extracted by the Folch technique from Langendorff-perfused rat hearts after administration of 15-( p-[ 131I]iodophenyl)pentadecanoic acid and 15-( p-[ 125I]iodophenyl)-3-R,S-methylpentadecanoic acid. Techniques utilizing successive high performance liquid chromatographic (HPLC) analyses have been developed for the evaluation of the uptake of the tracers into neutral lipids and phospholipids of the rat hearts. Phospholipids were separated on a SiO 2 column eluted with a gradient of acetonitrile/water ( 97.5 2.5 ) and acetonitrile/water ( 85 15 ) followed by separation of the neutral lipids on a C-18 reversed phase column with a gradient consisting of acetonitrile and 2-propanol/hexane ( 60 40 ) containing 1 N H 2SO 4 (5μL/100 mL). Both tracers show the incorporation into the expected major lipid classes.