Influence of temperature, pH and water activity on “in vitro” inhibition of Penicillium glabrum (Wehmer) Westling by yeasts

• Published in: Microbiological research Vol. 153; no. 2; pp. 137 - 143
• Main Authors: Sinigaglia, Milena, Rosaria Corbo, Maria, Ciccarone, Claudio
• Format: Journal Article
• Language: English
• Published: Basingstoke Elsevier GmbH 01.08.1998; Nature Publishing Group
• Subjects: Agar; Antibiosis; Biological and medical sciences; Biological control; Colony Count, Microbial; Control; Cryptococcus albidus; Ecology; Food Microbiology; Fundamental and applied biological sciences. Psychology; Fungal plant pathogens; Hydrogen-Ion Concentration; Kluyveromyces marxianus; Metschnikowia pulcherrima; modelling; Penicillium - growth & development; Penicillium glabrum; Pest Control, Biological; Phytopathology. Animal pests. Plant and forest protection; Rosales - microbiology; Saccharomycopsis vini; Temperature; Water; Yeasts - isolation & purification; Yeasts - physiology; modelling; biological control; Penicillium glabrum; Microorganism interrelationships; Temperature; Yeast; Plant pathogen; Biological control; Environmental factor; Modeling; Fungi; Antagonism; Water activity; Interspecific competition; pH; Kluyveromyces marxianus; Metschnikowia pulcherrima; Ascomycetes; Fungi Imperfecti; Mathematical model; Cryptococcus albidus; Thallophyta
• ISSN: 0944-5013; 1618-0623
• DOI: 10.1016/S0944-5013(98)80031-1

Four different yeast species ( Metschnikowia pulcherrima, Saccharomycopsis vini, Kluyveromyces marx ian us, Cryptococcus albidus), isolated from surface of grapes, were evaluated for biocontrol potential against Penicillium glabrum. In order to investigate the influence of temperature, pH, water activity and yeast cell concentration on Penicillium glabrum inhibition, the individual effects and the interaction of these factors were analyzed by means of a Central Composite Design (CCD). All yeast species tested showed antagonistic effects which were more pronounced at high cell concentrations. The other variables affected the antagonistic effect differentially depending on the yeast species. Results of the experimental design showed that the selective success of a competitive microflora is under environmental control; moreover, when microbial cells are subjected to multiple factors, the effects and the reciprocal interactions of the individual variables cannot be independently evaluated.