Detection of spring viraemia of carp virus (SVCV) with monoclonal antibodies

• Published in: Veterinární medicína Vol. 52; no. 7; pp. 308 - 316
• Main Authors: Reschova, S.,Vyzkumny Ustav Veterinarniho Lekarstvi, Brno (Czech Republic), Pokorova, D.,Vyzkumny Ustav Veterinarniho Lekarstvi, Brno (Czech Republic), Nevorankova, Z.,Vyzkumny Ustav Veterinarniho Lekarstvi, Brno (Czech Republic), Hulova, J.,Vyzkumny Ustav Veterinarniho Lekarstvi, Brno (Czech Republic), Vesely, T.,Vyzkumny Ustav Veterinarniho Lekarstvi, Brno (Czech Republic)
• Format: Journal Article
• Language: English
• Published: Prague Czech Academy of Agricultural Sciences (CAAS) 01.07.2007; Czech Academy of Agricultural Sciences
• Subjects: Absorbance; ANTICORPS MONOCLONAL; ANTICUERPOS MONOCLONALES; Assaying; Carp; Cross-reaction; Cross-reactivity; CYPRINIDAE; DIAGNOSIS; DIAGNOSTIC; DIAGNOSTICO; diagnostics; ELISA; Enzyme-linked immunosorbent assay; Esox; Fish diseases; FISH RHABDOVIRUSES; Glycoprotein G; Glycoproteins; http://www.fao.org/aos/agrovoc#c_15968; http://www.fao.org/aos/agrovoc#c_16464; http://www.fao.org/aos/agrovoc#c_2238; http://www.fao.org/aos/agrovoc#c_24051; http://www.fao.org/aos/agrovoc#c_2932; http://www.fao.org/aos/agrovoc#c_32817; http://www.fao.org/aos/agrovoc#c_40064; http://www.fao.org/aos/agrovoc#c_8260; IMMUNOBLOTTING; Immunoperoxidase test; Infectious hematopoietic necrosis virus; Infectious pancreatic necrosis; Infectious pancreatic necrosis virus; MONOCLONAL ANTIBODIES; Necrosis; Pancreas; RHABDOVIRUS DE LOS PECES; RHABDOVIRUS DES POISSONS; SEPTICAEMIA; SEPTICEMIA; SEPTICEMIE; spring viraemia of carp; svcv; TEST ELISA; Viral hemorrhagic septicemia virus; Viremia; VIROSE; VIROSES; VIROSIS; Viruses; Western blotting
• ISSN: 0375-8427; 1805-9392
• DOI: 10.17221/2043-VETMED

Monoclonal antibodies (MAbs) against spring viraemia of carp virus (SVCV), a severe disease in cyprinid fish, were prepared. Nine MAbs were characterised using Western blotting (WB). They all reacted with glycoprotein G, except for MAb 2E1 which showed no reactivity in WB. All nine MAbs showed specificity in an immunoperoxidase test. In ELISA assays, their titres ranged between 1:32 000 and 1:128 000. SVCV isolates from different European regions were examined by sandwich ELISA assay using the MAbs at a concentration of 15 microg/mL. Only MAb 4C12/3C8 showed low sensitivity in most of the isolates at the absorbance of A450. No cross-reaction with the infectious haematopoietic necrosis virus, viral haemorrhagic septicaemia virus or infectious pancreatic necrosis virus was demonstrated. 2E1 did not show cross-reactivity with pike fry rhabdovirus (PFRV) classified in genogroup III-IV, but reacted with a Czech SVCV isolate; its identity was confirmed by means of RT PCR assay. The other MAbs reacted positively with the PFRV F4 reference strain, isolated from Esox lucius (genogroup III).