Novosphingobium tardaugens sp. nov., an oestradiol-degrading bacterium isolated from activated sludge of a sewage treatment plant in Tokyo

• Published in: International journal of systematic and evolutionary microbiology Vol. 53; no. 1; pp. 47 - 52
• Main Authors: Fujii, Katsuhiko, Satomi, Masataka, Morita, Naoki, Motomura, Taizo, Tanaka, Tadaharu, Kikuchi, Shintaro
• Format: Journal Article
• Language: English
• Published: Reading Soc General Microbiol 01.01.2003; Society for General Microbiology
• Subjects: Alphaproteobacteria - classification; Alphaproteobacteria - genetics; Alphaproteobacteria - isolation & purification; Alphaproteobacteria - metabolism; Bacteriology; Base Composition; Biodegradation, Environmental; Biological and medical sciences; DNA, Bacterial - chemistry; DNA, Bacterial - genetics; DNA, Ribosomal - genetics; Estradiol - metabolism; Fatty Acids - analysis; Fundamental and applied biological sciences. Psychology; Lipids - analysis; Microbiology; Microscopy, Electron; Molecular Sequence Data; Novosphingobium tardaugens; Nucleic Acid Hybridization; Phylogeny; Quinones - analysis; RNA, Bacterial - genetics; RNA, Ribosomal, 16S - genetics; Sewage - microbiology; Sphingoglycolipids; Systematics; Tokyo; Tokyo; Japan; Activated sludge; Phenotype; Description; Estrogen; Bacteria; New species; Sex steroid hormone; Sewage sludge; Estradiol; Ovarian hormone; Sewage treatment plant
• ISSN: 1466-5026; 1466-5034
• DOI: 10.1099/ijs.0.02301-0

1 Department of Applied Chemistry, Muroran Institute of Technology, Mizumoto, Muroran 050-8585, Japan 2 Food Processing Division, National Research Institute of Fisheries Science, Fukuura, Kanazawa, Yokohama 236-8648, Japan 3 Research Institute of Biological Resources, National Institute of Advanced Industrial Science and Technology, Toyohira, Sapporo 062-8517, Japan 4 Muroran Marine Station, Field Science Centre for Northern Biosphere, Hokkaido University, Bokoi-minamimachi, Muroran 051-0003, Japan 5 Technical Research Laboratory, Kurabo Industries, Shimokida-cho, Neyagawa, Osaka 572-0823, Japan Correspondence Katsuhiko Fujii kfu{at}mmm.muroran-it.ac.jp An oestradiol-degrading bacterium isolated at a sewage treatment plant in Tokyo was studied phenotypically, genotypically and phylogenetically. Analysis of its 16S rDNA sequence, DNA base composition, whole-cell fatty acid profile and isoprenoid quinone composition, as well as the presence of sphingoglycolipid, revealed that the isolate is a member of the genus Novosphingobium . However, the sequence similarity of its 16S rDNA to those of known Novosphingobium species was no higher than 97 %, implying that the isolate is distinctive. The results of DNA–DNA hybridization experiments and physiological characterization also indicated that the isolate represents a novel Novosphingobium species, for which the name Novosphingobium tardaugens sp. nov. is proposed; strain ARI-1 T (=JCM 11434 T =ATCC BAA-531 T =IFO 16725 T ) is the type strain. Abbreviations: E2, 17 -oestradiol; FAME, fatty acid methyl ester The DDBJ accession number for the 16S rDNA sequence of strain ARI-1 T is AB070237.