A device for extraction, manipulation and stretching of DNA from single human chromosomes

We describe the structure and operation of a micro/nanofluidic device in which individual metaphase chromosomes can be isolated and processed without being displaced during exchange of reagents. The change in chromosome morphology as a result of introducing protease into the device was observed by t...

Full description

Saved in:
Bibliographic Details
Published inLab on a chip Vol. 11; no. 8; pp. 1431 - 1433
Main Authors Rasmussen, Kristian H, Marie, Rodolphe, Lange, Jacob M, Svendsen, Winnie E, Kristensen, Anders, Mir, Kalim U
Format Journal Article
LanguageEnglish
Published England 01.01.2011
Subjects
Chromosomes
Chromosomes, Human
Diffusion
DNA - chemistry
DNA - isolation & purification
DNA - metabolism
Elasticity
Equipment Design
Humans
Metaphase
Microfluidic Analytical Techniques - instrumentation
Nanotechnology - instrumentation
Peptide Hydrolases - metabolism
Online AccessGet full text

Cover

More Information
Summary:We describe the structure and operation of a micro/nanofluidic device in which individual metaphase chromosomes can be isolated and processed without being displaced during exchange of reagents. The change in chromosome morphology as a result of introducing protease into the device was observed by time-lapse imaging; pressure-driven flow was then used to shunt the chromosomal DNA package into a nanoslit. A long linear DNA strand (>1.3 Mbp) was seen to stretch out from the DNA package and along the length of the nanoslit. Delivery of DNA in its native metaphase chromosome package as well as the microfluidic environment prevented DNA from shearing and will be important for preparing ultra-long lengths of DNA for nanofluidic analysis.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ObjectType-Article-2
ObjectType-Feature-1
ISSN:1473-0197
1473-0189
DOI:10.1039/c0lc00603c