Transport and attenuation of Salmonella enterica, fecal indicator bacteria and a poultry litter marker gene are correlated in soil columns

• Published in: The Science of the total environment Vol. 598; pp. 204 - 212
• Main Authors: Mantha, Sirisha, Anderson, Angela, Acharya, Saraswati Poudel, Harwood, Valerie J., Weidhaas, Jennifer
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 15.11.2017
• Subjects: Animals; DNA, Bacterial - isolation & purification; Fecal indicator bacteria; Feces - microbiology; Genes, Bacterial; Groundwater - microbiology; Microbial source tracking; Pathogens; Poultry - microbiology; Poultry litter; Quantitative PCR; Salmonella enterica; Soil; Soil column studies; Soil Microbiology; Transport; Soil column studies; Pathogens; Microbial source tracking; Fecal indicator bacteria; Transport; Quantitative PCR; Poultry litter
• ISSN: 0048-9697; 1879-1026
• DOI: 10.1016/j.scitotenv.2017.04.020

Millions of tons of fecal-contaminated poultry litter are applied to U.S. agricultural fields annually. Precipitation and irrigation facilitate transport of fecal-derived pathogens and fecal indicator bacteria (FIB) to groundwater. The goal of this study was to compare transport of pathogens, FIB, and a microbial source tracking marker gene for poultry litter (LA35) in a simulated soil-to-groundwater system. Nine laboratory soil columns containing four different soil types were used to evaluate microbial transport to groundwater via infiltration. Quantitative polymerase chain reaction was used to monitor Salmonella enterica Typhimurium, Escherichia coli, Enterococcus spp., Brevibacterium sp. LA35 and Bacteroidales leached from soil columns inoculated with poultry litter. S. enterica was correlated with LA35 poultry litter marker gene and FIB concentrations in column soils containing organic matter, but not in acid washed sands. In contrast, S. enterica was found to correlate with LA35 and FIB in the leachate from columns containing sand, but not with leachate from organic soil columns. The majority of recovered DNA was found in leachate of predominately sandy soil columns, and in the soil of loamy columns. At least 90% of the DNA retained in soils for each microbial target was found in the top 3cm of the column. These studies suggest that poultry litter associated pathogens and FIB are rapidly released from litter, but are influenced by complex attenuation mechanisms during infiltration, including soil type. This study advances our understanding of the potential for subsurface transport of poultry litter associated pathogens and FIB, and support the use of the LA35 marker gene for evaluating poultry litter impacts on groundwater. [Display omitted] •Pathogens and FIB are released from poultry litter during rainfall and irrigation.•S. enterica in soil leachate correlates with a poultry litter marker gene and FIB.•Microorganisms decline 2 to 9 log gene copies (g soil)−1 within 12in. of soil.