Growth inhibitory effects of 5-aza-2′-deoxycytidine in HL-60 promyelocytic leukemia cells resistant to differentiation induction

• Published in: Biochemical and biophysical research communications Vol. 141; no. 2; pp. 629 - 635
• Main Authors: Levva, Albert, Schwartsmann, Gilberto, Boeije, Leonie C.M., Pinedo, Herbert M., de Waal, Frans
• Format: Journal Article
• Language: English
• Published: San Diego, CA Elsevier Inc 15.12.1986; Elsevier
• Subjects: Applied sciences; Azacitidine - analogs & derivatives; Azacitidine - pharmacology; Cell Cycle - drug effects; Cell Differentiation - drug effects; Cell Division - drug effects; Cell Line; Decitabine; Dimethyl Sulfoxide - pharmacology; Exact sciences and technology; Humans; Nitroblue Tetrazolium - metabolism; Other techniques and industries; Tretinoin - pharmacology; NBT; 5-aza-dCyd; nitroblue tetrazolium dye; 5-aza-2′-deoxycytidine
• ISSN: 0006-291X; 1090-2104
• DOI: 10.1016/S0006-291X(86)80219-4

In the original HL-60 cells (HL-60-S) and an HL-60 subline (HL-60-R) respectively susceptible and resistant to induction of differentiation by retinoic acid or dimethyl sulfoxide, 5-aza-2′-deoxycytidine inhibited growth equally but induced differentiation to a greater extent in HL-60-S. Flow cytometry showed that 5-aza-2′-deoxycytidine produced in both HL-60 lines an increased proportion of cells in G2+M rather than G0/Gl as with retinoic acid. 5-aza-2′-deoxycytidine may have a differentiation-inducing effect in HL-60 provided cells have the competence to differentiate, indicating the importance of an alternate mechanism of action.