Development of an all-in-one technology for glycan profiling targeting formalin-embedded tissue sections

• Published in: Biochemical and biophysical research communications Vol. 370; no. 2; pp. 259 - 263
• Main Authors: Matsuda, Atsushi, Kuno, Atsushi, Ishida, Hiroyasu, Kawamoto, Toru, Shoda, Jun-ichi, Hirabayashi, Jun
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 30.05.2008
• Subjects: Adenocarcinoma - chemistry; Biomarker; Biomarkers - analysis; Cell Line, Tumor; Colon cancer; Colonic Neoplasms - chemistry; Dissection; Formaldehyde - chemistry; Formalin-embedded; Glycan profiling; Humans; Lectin microarray; Lectins - chemistry; Plant Lectins - chemistry; Polysaccharides - analysis; Receptors, N-Acetylglucosamine - chemistry; Tissue array; Tissue Array Analysis - methods; Tissue Embedding; PBS; Biomarker; Lectin microarray; TBS; DAB; Formalin-embedded; Tissue array; Sia; GalNAc; BSA; SE; Gal; Colon cancer; NP-40; Glycan profiling; GlcNAc; MUC

An ultra-sensitive method for glycan analysis targeting small tissue sections (1.5mm in diameter) is described as an application of a recently-established lectin microarray technology. The developed system achieved a high level of detection of a tissue section consisting of approximately 500 cells f...