Development of an all-in-one technology for glycan profiling targeting formalin-embedded tissue sections

• Published in: Biochemical and biophysical research communications Vol. 370; no. 2; pp. 259 - 263
• Main Authors: Matsuda, Atsushi, Kuno, Atsushi, Ishida, Hiroyasu, Kawamoto, Toru, Shoda, Jun-ichi, Hirabayashi, Jun
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 30.05.2008
• Subjects: Adenocarcinoma - chemistry; Biomarker; Biomarkers - analysis; Cell Line, Tumor; Colon cancer; Colonic Neoplasms - chemistry; Dissection; Formaldehyde - chemistry; Formalin-embedded; Glycan profiling; Humans; Lectin microarray; Lectins - chemistry; Plant Lectins - chemistry; Polysaccharides - analysis; Receptors, N-Acetylglucosamine - chemistry; Tissue array; Tissue Array Analysis - methods; Tissue Embedding; PBS; Biomarker; Lectin microarray; TBS; DAB; Formalin-embedded; Tissue array; Sia; GalNAc; BSA; SE; Gal; Colon cancer; NP-40; Glycan profiling; GlcNAc; MUC
• ISSN: 0006-291X; 1090-2104
• DOI: 10.1016/j.bbrc.2008.03.090

An ultra-sensitive method for glycan analysis targeting small tissue sections (1.5mm in diameter) is described as an application of a recently-established lectin microarray technology. The developed system achieved a high level of detection of a tissue section consisting of approximately 500 cells for differential profiling, where both N- and O-glycans attached to a pool of glycoproteins are subjected to multiplex analysis with 43 lectins. By using an optimized protocol for differential glycan analysis, sections of adenocarcinoma (n=28) and normal epithelia (n=12) of the colon were analyzed in an all-in-one manner. As a result, Wisteria floribunda agglutinin (WFA) was found to clearly differentiate cancerous from normal epithelia with P<0.0001. The obtained results correlated well with the subsequent histochemical study using biotinylated WFA. Thus, the developed technology proved to be valid for expanding the lectin microarray applications to tissue-based glycomics, and hence, should accelerate a discovery phase of glycan-related biomarkers.