Simultaneous determination of triazole fungicides in animal-origin food by ultra-high-performance liquid chromatography coupled with tandem mass spectrometry

• Published in: Food Chemistry: X Vol. 20; p. 100956
• Main Authors: Xing, Lijie, Liu, Yang, Li, Wenqi, Zou, Liangjun, Wang, Yuan, Luo, Ruifeng
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier Ltd 30.12.2023; Elsevier
• Subjects: Animal-origin foods; QuEChERS; Triazole fungicide; UPLC-MS/MS; UPLC-MS/MS; Animal-origin foods; Triazole fungicide; QuEChERS
• ISSN: 2590-1575; 2590-1575
• DOI: 10.1016/j.fochx.2023.100956

•The methods of 21 triazole pesticide residues in animal-origin foods were established.•A combined modified QuEChERS and LC-MS/MS method was optimized.•This method is simple, sensitive, rapid, practical and accurate.•No triazole pesticide residues have been found in animal-origin foods. A method for the simultaneous determination of 21 triazole fungicides in animal-origin foods was established by using UPLC–MS/MS. The dilution solvent, extraction solvent, and QuEChERS purification adsorbent composition, were optimized. The response value of the target compound was the highest and the chromatographic peak shape was optimal under the following conditions: water-acetonitrile as the mobile phase, acetonitrile to extract the target compound, C18 (100 mg) as the adsorbent, and water-acetonitrile as the diluent. Our method was validated under electrospray ionization (ESI) + conditions with six animal-origin foods. The 21 triazole fungicides showed good linear relationships (0.1–20 μg∙L−1, R2 > 0.99). The limits of detection and quantitation ranged from 0.1 to 0.3 μg∙kg−1 and 0.3 to 0.9 μg∙kg−1, respectively. The average recoveries ranged from 72.0% to 114.8% with RSDs < 9.9%. Therefore, our method was suitable for the determination of pesticide residues in commercially available animal-origin samples.