Differential effects of PDGF and PDGF-BB on vascular smooth muscle cells

Several biological effects of recombinant PDGF-BB and PDGF obtained from human platelets were examined with vascular smooth muscle cells. Although PDGF and PDGF-BB were equally potent mitogens for these cells, 5 fold higher levels of PDGF were required to displace 125I-PDGF-BB binding than PDGF-BB i...

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Bibliographic Details
Published inBiochemical and biophysical research communications Vol. 160; no. 3; pp. 1047 - 1054
Main Authors Reilly, Christopher F., Broski, Jill E.
Format Journal Article
LanguageEnglish
Published San Diego, CA Elsevier Inc 15.05.1989
Elsevier
Subjects
Analytical, structural and metabolic biochemistry
Animals
Aorta, Thoracic
Binding, Competitive
Biological and medical sciences
Blood Platelets - analysis
Cattle
DNA - biosynthesis
Fundamental and applied biological sciences. Psychology
Humans
Immunosorbent Techniques
Macromolecular Substances
Membrane Proteins - metabolism
Molecular Weight
Muscle, Smooth, Vascular - metabolism
Phosphorylation
Phosphotyrosine
Platelet-Derived Growth Factor - metabolism
Platelet-Derived Growth Factor - pharmacology
Protein hormones. Growth factors. Cytokines
Proteins
Radioligand Assay
Recombinant Proteins - pharmacology
Tyrosine - analogs & derivatives
Tyrosine - metabolism
CS
hSMC
SDS
EGF
PDGF
bSMC
PAGE
K
DME
PDS
Human
Ligand binding
Phosphorylation
Recombination
Radiolabelling
Gel electrophoresis
Smooth muscle
Biological activity
Blood
Blood cell
Platelet
Blood vessel
Mitogen
Comparative study
Platelet derived growth factor
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Summary:Several biological effects of recombinant PDGF-BB and PDGF obtained from human platelets were examined with vascular smooth muscle cells. Although PDGF and PDGF-BB were equally potent mitogens for these cells, 5 fold higher levels of PDGF were required to displace 125I-PDGF-BB binding than PDGF-BB itself. Higher concentrations of PDGF relative to PDGF-BB were also required to stimulate the phosphorylation of a 163K protein in membrane preparations. PDGF-BB, but not PDGF, treatment of intact cells resulted in the phosphorylation on tyrosine residues of 168, 53, 48, and 45K proteins. The data suggest that PDGF and PDGF-BB stimulate smooth muscle cell mitogenesis by different mechanisms.
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ISSN:0006-291X
1090-2104
DOI:10.1016/S0006-291X(89)80108-1