Metabolic rate and reactive oxygen species production in different genotypes of GH-transgenic zebrafish

• Published in: Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology Vol. 149; no. 1; pp. 209 - 214
• Main Authors: Rosa, C.E., Figueiredo, M.A., Lanes, C.F.C., Almeida, D.V., Monserrat, J.M., Marins, L.F.
• Format: Journal Article
• Language: English
• Published: England Elsevier Inc 2008
• Subjects: Actins - genetics; Actins - metabolism; Animals; Animals, Genetically Modified - genetics; Animals, Genetically Modified - metabolism; Antioxidants - metabolism; Catalytic Domain - genetics; Glutamate-Cysteine Ligase - biosynthesis; Glutamate-Cysteine Ligase - genetics; Glutamate-cysteine ligase catalytic subunit; Glutathione - biosynthesis; Glutathione - genetics; Growth hormone; Growth Hormone - biosynthesis; Growth Hormone - genetics; Lipid Peroxidation - genetics; Oxygen consumption; Oxygen Consumption - genetics; Promoter Regions, Genetic - genetics; Reactive oxygen species; Reactive Oxygen Species - metabolism; Transgenic zebrafish; Zebrafish; Zebrafish Proteins - biosynthesis; Zebrafish Proteins - genetics; Glutamate-cysteine ligase catalytic subunit; Oxygen consumption; Reactive oxygen species; Transgenic zebrafish; Growth hormone
• ISSN: 1096-4959; 1879-1107
• DOI: 10.1016/j.cbpb.2007.09.010

Growth hormone overexpression increases growth and consequently increases the metabolic rate in fishes. Therefore, the objective of this study was to evaluate the effects of growth hormone overexpression in zebrafish Danio rerio in terms of growth, oxygen consumption, reactive oxygen species production, lipid hydroperoxide content, antioxidant enzyme activity and glutamate-cysteine ligase catalytic subunit gene expression. The employed models were wild type and transgenic (hemizygous and homozygous) zebrafish expressing the Odonthestes argentinensis growth hormone gene directed by the Cyprinus carpio beta-actin promoter. Higher growth parameters were observed in the hemizygous group. The homozygous group possessed higher oxygen consumption and reactive oxygen species production. Growth hormone transgenesis causes a decrease in glutamate-cysteine ligase catalytic subunit expression, an enzyme responsible for glutathione synthesis. Although the lipid hydroperoxide content was similar between groups, we demonstrate that growth hormone overexpression has the potential to generate oxidative stress in fishes.