Adsorption and viscoelastic properties of fractionated mucin (BSM) and bovine serum albumin (BSA) studied with quartz crystal microbalance (QCM-D)

• Published in: Journal of colloid and interface science Vol. 315; no. 2; pp. 475 - 481
• Main Authors: Feiler, Adam A., Sahlholm, Anna, Sandberg, Tomas, Caldwell, Karin D.
• Format: Journal Article
• Language: English
• Published: San Diego, CA Elsevier Inc 15.11.2007; Elsevier
• Subjects: Adsorption; Animals; Biocompatible Materials - chemistry; Biomaterial; Bovine serum albumin (BSA); Bovine submaxillary gland mucin (BSM); Cattle; Chemistry; Coated Materials, Biocompatible - chemistry; Colloidal state and disperse state; Elasticity; Exact sciences and technology; General and physical chemistry; Kemi; Materials Testing; Mucins - chemistry; NATURAL SCIENCES; NATURVETENSKAP; Polystyrenes; Protein; Quartz; Quartz crystal microbalance with dissipation monitoring (QCM-D); Serum Albumin, Bovine - chemistry; Surface physical chemistry; Surface Properties; Viscoelastic; Viscosity; Bovine serum albumin (BSA); Biomaterial; Bovine submaxillary gland mucin (BSM); Viscoelastic; Protein; Quartz crystal microbalance with dissipation monitoring (QCM-D); Viscoelasticity; Bovine; Crystals; Serum albumin; Vertebrata; Mammalia; Adsorption; Artiodactyla; Ungulata; Rheological properties; Quartz microbalance
• ISSN: 0021-9797; 1095-7103; 1095-7103
• DOI: 10.1016/j.jcis.2007.07.029

The adsorption profile and viscoelastic properties of bovine submaxillary gland mucin (BSM) and bovine serum albumin (BSA), extracted from a commercial mucin preparation, adsorbing to polystyrene surfaces has been studied using quartz crystal microbalance with dissipation monitoring (QCM-D). A significant difference in the adsorption properties of the different proteins was detected; with the BSA adsorbing in a flat rigid layer whilst the mucin adsorbed in a diffuse, highly viscoelastic layer. Subsequent addition of BSA to the preadsorbed mucin layer resulted in stiffening of the protein layer which was attributed to complexation of the mucin by BSA. In contrast, a preadsorbed layer of BSA prevented mucin adsorption altogether. Combined mixtures of mucin and BSA in well defined ratios revealed intermediate properties between the two separate protein species which varied systematically with the protein ratios. The results shed light on the synergistic effects of complexation of lower molecular weight biomolecular species with mucin. The possibility to selectively control protein uptake and tailor the physical properties of the adsorbed layer makes mucin an attractive option for application in biomaterial coatings. Viscoelastic properties of adsorbed layers mucin and albumin complexes measured using quartz crystal microbalance with dissipation monitoring (QCM-D); potential coating strategy for biomaterials.