DNA bending is induced by binding of the glucocorticoid receptor DNA binding domain and progesterone receptors to their response element

• Published in: The Journal of steroid biochemistry and molecular biology Vol. 60; no. 1; pp. 31 - 41
• Main Authors: Petz, Larry N., Nardulli, Ann M., Kim, Jongsook, Horwitz, Kathryn B., Freedman, Leonard P., Shapiro, David J.
• Format: Journal Article
• Language: English
• Published: Oxford Elsevier Ltd 1997; Elsevier Science
• Subjects: Binding Sites; Biological and medical sciences; Dimerization; DNA - chemistry; DNA - metabolism; Fundamental and applied biological sciences. Psychology; Humans; Nucleic Acid Conformation; Plasmids - chemistry; Plasmids - genetics; Receptors, Glucocorticoid - chemistry; Receptors, Glucocorticoid - genetics; Receptors, Glucocorticoid - metabolism; Receptors, Progesterone - chemistry; Receptors, Progesterone - genetics; Receptors, Progesterone - metabolism; Regulatory Sequences, Nucleic Acid; Steroid hormones. Cholecalciferol derivatives; Vertebrates: endocrinology; Human; DNA; Bending; Binding site; Progesterone; Sex steroid hormone; Response element; Glucocorticoid; Ovarian hormone; Induced conformation; Hormonal receptor
• ISSN: 0960-0760; 1879-1220
• DOI: 10.1016/S0960-0760(96)00171-9

Circular permutation analysis was used to determine the degree of DNA bending induced by binding of the glucocorticoid receptor (GR) DNA binding domain (DBD), the human progesterone receptor (PR) DBD, PR-A:A and PR-B:B homodimers, and PR-A:B heterodimers to the glucocorticoid response element/progesterone response element ( GRE PRE ). The bending angles induced by the GR DBD and the PR DBD were approximately 28° and 25°, respectively. The PR-B:B and PR-A:A homodimers and the PR-A:B heterodimers all induced similar DNA bending angles of 72–77°. The substantially greater DNA bend induced by full-length PR compared to the PR DBD indicates that sequences outside the classic zinc finger DNA binding domain may play an important role in the interaction of PR with the GRE PRE . Because PR-A:A and PR-B:B homodimers and the PR-A:B heterodimers induce similar DNA bends, the different abilities of the PR-A and PR-B isoforms to activate transcription are not due to differences in their abilities to distort DNA structure.