Human tissue-resident peritoneal macrophages reveal resistance towards oxidative cell stress induced by non-invasive physical plasma

• Published in: Frontiers in immunology Vol. 15; p. 1357340
• Main Authors: Schultze-Rhonhof, Laura, Marzi, Julia, Carvajal Berrio, Daniel Alejandro, Holl, Myriam, Braun, Theresa, Schäfer-Ruoff, Felix, Andress, Jürgen, Bachmann, Cornelia, Templin, Markus, Brucker, Sara Y, Schenke-Layland, Katja, Weiss, Martin
• Format: Journal Article
• Language: English
• Published: Switzerland Frontiers Media S.A 05.03.2024
• Subjects: cold atmospheric plasma (CAP); human primary macrophages; Humans; immune response; Immunology; Macrophages; Macrophages, Peritoneal; non-invasive physical plasma (NIPP); Oxidative Stress; Peritoneal Cavity; Peritoneal Neoplasms - metabolism; Peritoneum - metabolism; plasma-activated media (PAM); plasma-treated solutions (PTS); plasma-activated media (PAM); non-invasive physical plasma (NIPP); immune response; human primary macrophages; peritoneal cancer; cold atmospheric plasma (CAP); peritoneal cavity; plasma-treated solutions (PTS)
• ISSN: 1664-3224; 1664-3224
• DOI: 10.3389/fimmu.2024.1357340

In the context of multimodal treatments for abdominal cancer, including procedures such as cytoreductive surgery and intraperitoneal chemotherapy, recurrence rates remain high, and long-term survival benefits are uncertain due to post-operative complications. Notably, treatment-limiting side effects often arise from an uncontrolled activation of the immune system, particularly peritoneally localized macrophages, leading to massive cytokine secretion and phenotype changes. Exploring alternatives, an increasing number of studies investigated the potential of plasma-activated liquids (PAL) for adjuvant peritoneal cancer treatment, aiming to mitigate side effects, preserve healthy tissue, and reduce cytotoxicity towards non-cancer cells. To assess the non-toxicity of PAL, we isolated primary human macrophages from the peritoneum and subjected them to PAL exposure. Employing an extensive methodological spectrum, including flow cytometry, Raman microspectroscopy, and DigiWest protein analysis, we observed a pronounced resistance of macrophages towards PAL. This resistance was characterized by an upregulation of proliferation and anti-oxidative pathways, countering PAL-derived oxidative stress-induced cell death. The observed cellular effects of PAL treatment on human tissue-resident peritoneal macrophages unveil a potential avenue for PAL-derived immunomodulatory effects within the human peritoneal cavity. Our findings contribute to understanding the intricate interplay between PAL and macrophages, shedding light on the promising prospects for PAL in the adjuvant treatment of peritoneal cancer.