Regulatory Effects of Stevia Rebaudiana on NADPH Oxidase-Related Manifestations of Oxidative Stress in Diabetic Rats with Spinal Cord Injury

• Published in: Neurophysiology (New York) Vol. 53; no. 1; pp. 13 - 21
• Main Authors: Simonyan, K. V., Chavushyan, V. A., Avetisyan, L. G., Simonyan, R. M., Isoyan, A. S., Simonyan, G. M., Hovhannisyan, L. E., Simonyan, M. A.
• Format: Journal Article
• Language: English
• Published: New York Springer US 2021; Springer; Springer Nature B.V
• Subjects: Antioxidants; Biomedical and Life Sciences; Biomedicine; Bone marrow; CYBB protein; Diabetes mellitus; Fructose; Isoforms; NAD(P)H oxidase; Neurosciences; Oxidative stress; Rodents; Spinal cord injuries; Stevia; spinal cord injury; diabetic rats; NADPH-containing lipoprotein associates (NCL); Suprol; Nox isoforms; phytopreparation; oxidative stress
• ISSN: 0090-2977; 1573-9007
• DOI: 10.1007/s11062-021-09908-2

For the first time, a method for isolation and purification of Nox isoforms (Nox1+Nox2), as well as of O 2 – -producing stable associates of Nox and lipoprotein-containing NADPH isoforms (NCL associates), from the membranes of cells and intracellular formations of the spinal cord (SC) and bone marrow (BM) of albino rats was developed, and the respective estimates were obtained. The effects of treatment with a phytopreparation of Stevia (Stevia rebaudiana Bertoni ) leaves in this pathology were studied in a model of fructose-induced type II diabetes (FID) in rats with spinal cord injury (SCI). Due to its antioxidant activity, Stevia exhibited a clear membrane-stabilizing effect in rats with FID and SC hemisection. In such experimental animals, the qualitative and quantitative indices for Nox isoforms and NCL associates from the SC and BM membranes showed clear trends toward normalization.