Image analysis of two-dimensional gel electrophoresis for comparative proteomics of transgenic and non-transgenic soybean seeds
Considering the importance of bidimensional electrophoresis and image analysis in comparative proteomics, the parameters that influence the analysis of protein expression of transgenic and non-transgenic soybean seeds were evaluated. The loaded mass of the proteins (150–500µg), the pH separation ran...
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Published in | Journal of proteomics Vol. 73; no. 8; pp. 1433 - 1440 |
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Main Authors | , , |
Format | Journal Article Conference Proceeding |
Language | English |
Published |
Kidlington
Elsevier B.V
16.06.2010
Elsevier |
Subjects | |
Online Access | Get full text |
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Summary: | Considering the importance of bidimensional electrophoresis and image analysis in comparative proteomics, the parameters that influence the analysis of protein expression of transgenic and non-transgenic soybean seeds were evaluated. The loaded mass of the proteins (150–500µg), the pH separation range (3–10 or 4–7), and manual/automatic image editing were evaluated. Additionally, after optimizing the conditions, histograms and matchings were obtained in order to accurately analyze the variations (90%) in protein expression. From this, 10 proteins displayed significant differences in expression, and eight of them were characterized and identified by mass spectrometry.
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 1874-3919 1876-7737 |
DOI: | 10.1016/j.jprot.2010.01.009 |