Image analysis of two-dimensional gel electrophoresis for comparative proteomics of transgenic and non-transgenic soybean seeds

• Published in: Journal of proteomics Vol. 73; no. 8; pp. 1433 - 1440
• Main Authors: Brandão, A.R., Barbosa, H.S., Arruda, M.A.Z.
• Format: Journal Article Conference Proceeding
• Language: English
• Published: Kidlington Elsevier B.V 16.06.2010; Elsevier
• Subjects: 2D PAGE; Biological and medical sciences; Biomarkers; Diverse techniques; Electrophoresis, Gel, Two-Dimensional - methods; Fundamental and applied biological sciences. Psychology; Genetically modified organisms; Glycine max - chemistry; Hydrogen-Ion Concentration; Image Processing, Computer-Assisted - methods; Molecular and cellular biology; Plants, Genetically Modified; Proteomics - methods; Seeds - chemistry; Soybean; Soybean Proteins - chemistry; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - methods; Biomarkers; Soybean; 2D PAGE; Genetically modified organisms; Image analysis; Organism; Seeds; Proteomics; Biological marker; Two dimensional electrophoresis
• ISSN: 1874-3919; 1876-7737
• DOI: 10.1016/j.jprot.2010.01.009

Considering the importance of bidimensional electrophoresis and image analysis in comparative proteomics, the parameters that influence the analysis of protein expression of transgenic and non-transgenic soybean seeds were evaluated. The loaded mass of the proteins (150–500µg), the pH separation range (3–10 or 4–7), and manual/automatic image editing were evaluated. Additionally, after optimizing the conditions, histograms and matchings were obtained in order to accurately analyze the variations (90%) in protein expression. From this, 10 proteins displayed significant differences in expression, and eight of them were characterized and identified by mass spectrometry. [Display omitted]