Principal involvement of cyclooxygenase-1-derived prostaglandins in the c- fos expression of the rat hind brain following visceral stimulation with acetic acid

• Published in: Brain research. Molecular brain research. Vol. 52; no. 1; pp. 151 - 156
• Main Authors: Kusuhara, Hidenobu, Fukunari, Atsushi, Matsuyuki, Hirofumi, Okumoto, Takeki
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 01.12.1997; Elsevier
• Subjects: Acetic Acid - pharmacology; Animals; Biological and medical sciences; c -fos; Cyclooxygenase; Cyclooxygenase 1; Female; Fundamental and applied biological sciences. Psychology; Isoenzymes - physiology; Membrane Proteins; Mofezolac; mRNA expression; Nociception; Non-steroidal anti-inflammatory drug; NS-398; Prostaglandin; Prostaglandin-Endoperoxide Synthases - physiology; Prostaglandins - biosynthesis; Proto-Oncogene Proteins c-fos - biosynthesis; Rats; Rats, Sprague-Dawley; Rhombencephalon - drug effects; Rhombencephalon - metabolism; Somesthesis and somesthetic pathways (proprioception, exteroception, nociception); interoception; electrolocation. Sensory receptors; Stimulation, Chemical; Vertebrates: nervous system and sense organs; Viscera - drug effects; Viscera - metabolism; Nociception; Mofezolac; c -fos; Cyclooxygenase; Prostaglandin; Non-steroidal anti-inflammatory drug; NS-398; mRNA expression; Prostaglandin-endoperoxide synthase; Noxious stimulus; Rat; Enzyme; Arachidonic acid derivatives; Rodentia; Central nervous system; Gene expression; Vertebrata; Mammalia; Eicosanoid; Oxidoreductases; Protooncogene; Brain (vertebrata); Immediate early gene; Viscus
• ISSN: 0169-328X; 1872-6941
• DOI: 10.1016/S0169-328X(97)00263-5

We investigated the relationship between c- fos expression in the hind brain and peritoneal prostaglandin (PG) synthesis after visceronociceptive stimulation with acetic acid in rats. Time-course studies on the mRNA expression for c- fos in the hind brain and cyclooxygenase (COX) isoforms in the peritoneal cells, as well as on the peritoneal 6-keto-PGF 1 α accumulation, after stimulation indicated that COX-1 but not COX-2 was responsible for the peritoneal synthesis of PGs which were suggested to evoke c- fos expression in the hind brain. Pharmacological experiments using mofezolac, a preferential inhibitor against COX-1, and NS-398, a selective inhibitor against COX-2, confirmed the involvement of COX-1 derived PGs in the induction of c- fos expression in the hind brain following the noxious stimulation.