Transition metal ions within human atherosclerotic lesions can catalyse the oxidation of low density lipoprotein by macrophages

• Published in: FEBS letters Vol. 374; no. 1; pp. 12 - 16
• Main Authors: Lamb, David J., Mitchinson, Malcolm J., Leake, David S.
• Format: Journal Article
• Language: English
• Published: England Elsevier B.V 23.10.1995
• Subjects: Animals; Arteriosclerosis - metabolism; Atherosclerosis; BHT, butylated hydroxytoluene; Catalysis - drug effects; Chelating Agents - pharmacology; DMEM, Dulbecco's modified Eagle's medium; Female; Gruel; Humans; In Vitro Techniques; Ions; LDL, low density lipoprotein; Lipoproteins, LDL - metabolism; Low density lipoprotein; Macrophage; Macrophages - metabolism; MDA, malondialdehyde; Metals - metabolism; Mice; Oxidation-Reduction; Oxidised low density lipoprotein; Oxidized low density lipoprotein; Resins, Synthetic; TBARS, thiobarbituric acid-reactive substances; LDL, low density lipoprotein; Oxidized low density lipoprotein; DMEM, Dulbecco's modified Eagle's medium; MDA, malondialdehyde; Atherosclerosis; BHT, butylated hydroxytoluene; TBARS, thiobarbituric acid-reactive substances; Low density lipoprotein; Oxidised low density lipoprotein; Gruel; Macrophage
• ISSN: 0014-5793; 1873-3468
• DOI: 10.1016/0014-5793(95)01068-P

The oxidation of low density lipoprotein (LDL) in the arterial wall may contribute to atherogenesis. The oxidation of LDL by cells usually requires catalytically active transition metal ions. We show here some that gruel samples from human advanced atherosclerotic lesions are capable of catalysing the oxidation of LDL by macrophages as measured by thiobarbituric acid-reactive substances, enhanced electrophoretic mobility and increased macrophage uptake. This catalysis could be inhibited by pretreatment of the gruel with Chelex-100, which binds transition metal ions. The presence of catalytically active transition metal ions in atherosclerotic lesions may help to explain why LDL oxidation occurs at these sites.