Insight into topological and functional relationships of cytochrome c oxidase subunit I of Saccharomyces cerevisiae by means of intragenic complementation

In yeast, revertants were selected from four respiratory deficient mutants carrying mutations in the cytochrome c oxidase subunit I gene. Intragenic second site mutations revealed amino acids which are functionally complementary to the original mutated position and may be in topological interaction...

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Bibliographic Details
Published inFEBS letters Vol. 321; no. 2; pp. 159 - 162
Main Authors Meunier, Brigitte, Coster, Françoise, Lemarre, Philippe, Colson, Anne-Marie
Format Journal Article
LanguageEnglish
Published England Elsevier B.V 26.04.1993
Subjects
Amino Acid Sequence
Base Sequence
Codon - genetics
Cytochrome c oxidase
Electron Transport Complex IV - chemistry
Electron Transport Complex IV - metabolism
Genes, Fungal
Genetic Complementation Test
Intragenic complementation
Introns
Macromolecular Substances
Models, Structural
Molecular Sequence Data
Mutagenesis, Site-Directed
Mutation
Protein Structure, Secondary
Saccharomyces cerevisiae - enzymology
Saccharomyces cerevisiae - genetics
Yeast
Cytochrome c oxidase
Models
Yeast
Intragenic complementation
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Summary:In yeast, revertants were selected from four respiratory deficient mutants carrying mutations in the cytochrome c oxidase subunit I gene. Intragenic second site mutations revealed amino acids which are functionally complementary to the original mutated position and may be in topological interaction with it. The results provide additional data in favour of the model proposed for the structure of the binuclear centre in proton-motive oxidases.
ISSN:0014-5793
1873-3468
DOI:10.1016/0014-5793(93)80099-G