Expression and Proliferation‐Promoting Role of Diversin in the Neuronally Committed Precursor Cells Migrating in the Adult Mouse Brain

The subventricular zone (SVZ) is the largest neurogenic region in the adult rodent brain. In the adult SVZ, unlike in the embryonic brain, neuronally committed precursor cells (neuroblasts) maintain their proliferative activity while migrating toward the olfactory bulb (OB), suggesting that they are...

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Published inStem cells (Dayton, Ohio) Vol. 28; no. 11; pp. 2017 - 2026
Main Authors Ikeda, Makiko, Hirota, Yuki, Sakaguchi, Masanori, Yamada, Osamu, Kida, Yasuyuki S., Ogura, Toshihiko, Otsuka, Takanobu, Okano, Hideyuki, Sawamoto, Kazunobu
Format Journal Article
LanguageEnglish
Published Hoboken Wiley Subscription Services, Inc., A Wiley Company 01.11.2010
Subjects
Animals
Animals, Newborn
Blotting, Western
Brain - cytology
Brain - metabolism
Cell Movement - genetics
Cell Movement - physiology
Cell Proliferation
Cytoskeletal Proteins - genetics
Cytoskeletal Proteins - metabolism
Diversin
Female
Genetic Vectors - genetics
Immunohistochemistry
Intracellular Signaling Peptides and Proteins - genetics
Intracellular Signaling Peptides and Proteins - metabolism
Mice
Mice, Inbred ICR
Neurogenesis
Neurons - cytology
Neurons - metabolism
Proliferation
Retroviridae - genetics
Stem Cells - cytology
Stem Cells - metabolism
Subventricular zone
Wnt signaling
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Summary:The subventricular zone (SVZ) is the largest neurogenic region in the adult rodent brain. In the adult SVZ, unlike in the embryonic brain, neuronally committed precursor cells (neuroblasts) maintain their proliferative activity while migrating toward the olfactory bulb (OB), suggesting that they are inhibited from exiting the cell cycle. Little is known about the mechanisms underlying the unique ability of adult neuroblasts to proliferate during migration. Here, we studied the expression and function of Diversin, a component of the Wnt signaling pathways. In the neonatal and adult mouse brain, Diversin expression was observed in neuroblasts and mature neurons in the SVZ and hippocampus. Retrovirus‐mediated overexpression of Diversin promoted the proliferation of neuroblasts and increased the number of neuroblasts that reached the OB. Conversely, the knockdown of Diversin decreased the proliferation of neuroblasts. Our results indicate that Diversin plays an important role in the proliferation of neuroblasts in the SVZ of the adult brain. STEM CELLS 2010;28:2017–2026
Bibliography:First published online in STEM CELLS
Author contributions: M.I.: conception and design, collection and assembly of data, data analysis and interpretation, manuscript writing; Y.H.: conception and design, data analysis and interpretation, manuscript writing; M.S.: conception and design, collection and assembly of data, data analysis and interpretation; O.Y.: conception and design, collection and assembly of data, data analysis and interpretation; Y.S.K.: conception and design, provision of study material or patients; T. Ogura: conception and design, provision of study material or patients; T. Otsuka: data analysis and interpretation, administrative support; H.O.: conception and design, data analysis and interpretation, financial support, administrative support; K.S.: conception and design, data analysis and interpretation, financial support, manuscript writing, administrative support.
September 8, 2010.
Disclosure of potential conflicts of interest is found at the end of this article.
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ISSN:1066-5099
1549-4918
DOI:10.1002/stem.516