Changes in PEDF, MMP-2, and TGF-β2 levels in the aqueous humor of cataract patients and their correlation with disease severity

• Published in: Clinics (São Paulo, Brazil) Vol. 79; p. 100402
• Main Authors: Lin, Yong Feng, Xie, Jin Xia, Chen, Xiao Luan
• Format: Journal Article
• Language: English
• Published: United States Elsevier España, S.L.U 01.01.2024; Hospital das Clinicas da Faculdade de Medicina da Universidade de Sao Paulo; Faculdade de Medicina / USP
• Subjects: Adult; Aged; Aqueous Humor; Aqueous Humor - chemistry; Aqueous Humor - metabolism; Biomarkers - analysis; Case-Control Studies; Cataract; Cataract - metabolism; Eye Proteins - analysis; Eye Proteins - metabolism; Female; Humans; LOCS III Classification; Male; Matrix Metalloproteinase 2 - analysis; Matrix Metalloproteinase 2 - metabolism; Matrix Metalloproteinase-2; MEDICINE, GENERAL & INTERNAL; Middle Aged; Nerve Growth Factors - analysis; Nerve Growth Factors - metabolism; Original; Pigment Epithelial-Derived Factor; Reference Values; ROC Curve; Serpins - analysis; Serpins - metabolism; Severity of Illness Index; Transforming Growth Factor beta2 - analysis; Transforming Growth Factor beta2 - metabolism; Transforming Growth Factor-β2; Aqueous Humor; LOCS III Classification; Cataract; Pigment Epithelial-Derived Factor; Matrix Metalloproteinase-2; Transforming Growth Factor-β2
• ISSN: 1807-5932; 1980-5322; 1980-5322
• DOI: 10.1016/j.clinsp.2024.100402

•PEDF levels were lower and MMP-2 and TGF-β2 levels were higher in cataracts.•The combined detection was valuable in evaluating cataract development.•MMP-2, TGF-β2, and PEDF were risk factors for cataract development. To explore the changes of Pigment Epithelium-Derived Factor (PEDF), Matrix Metalloproteinase-2 (MMP-2), and Transforming Growth Factor-β2 (TGF-β2) levels in the aqueous humor of cataract patients and their correlation with disease severity. 93 cataract patients and 56 healthy subjects were study objects. PEDF, MMP-2, and TGF-β2 levels of aqueous humor were compared, and the correlation between each index and Lens Opacity Classification System (LOCS) III classification was analyzed. ROC curve was used to analyze the evaluation value of the combined detection of each index on cataract development, and logistic regression to analyze the influence of the changes of each index on cataract development. PEDF levels were lower and MMP-2 and TGF-β2 levels were higher in the aqueous humor of cataract patients than in healthy subjects. PEDF levels in the aqueous humor were negatively correlated with LOCS III classification, while MMP-2 and TGF-β2 levels were positively correlated with LOCS III classification. The AUC value of combined detection was higher than that of PEDF, MMP-2, and TGF-β2 in the aqueous humor alone. MMP-2 ≥ 15.13 pg/mL, TGF-β2 ≥ 385.91 pg/mL and PEDF < 198.85 ng/mL were risk factors for cataract development. The changes in PEDF, MMP-2, and TGF-β2 levels in the aqueous humor of cataract patients are related to LOCS III classification. The combined detection is valuable in evaluating cataract development.