Surveillance of Adenovirus D in patients with epidemic keratoconjunctivitis from Fukui Prefecture, Japan, 1995-2010

• Published in: Journal of medical virology Vol. 84; no. 1; pp. 81 - 86
• Main Authors: Nakamura, Masako, Hirano, Eiko, Kowada, Kazuaki, Ishiguro, Fubito, Yamagishi, Zenya, Adhikary, Arun Kumar, Hanaoka, Nozomu, Okabe, Nobuhiko, Taniguchi, Kiyosu, Fujimoto, Tsuguto
• Format: Journal Article
• Language: English
• Published: Hoboken Wiley Subscription Services, Inc., A Wiley Company 01.01.2012; Wiley; Wiley Subscription Services, Inc
• Subjects: Adenoviridae Infections - epidemiology; Adenoviridae Infections - virology; Adenoviruses, Human - classification; Adenoviruses, Human - genetics; Adenoviruses, Human - isolation & purification; Biological and medical sciences; Fundamental and applied biological sciences. Psychology; Genotype; Human viral diseases; Humans; Infectious diseases; Japan - epidemiology; Keratoconjunctivitis - epidemiology; Keratoconjunctivitis - virology; loop-mediated isothermal amplification; Medical sciences; Microbiology; Miscellaneous; Molecular Sequence Data; Neutralization Tests; new type adenovirus; Nucleic Acid Amplification Techniques; outbreak; PCR-sequencing; Sequence Analysis, DNA; Viral diseases; Virology; Virus Cultivation; Japan; Human; Adenoviridae; new type adenovirus; Loop mediated isothermal amplification reaction; PCR-sequencing; Keratopathy; Conjunctiva disease; Infection; Virus; Polymerase chain reaction; Eye disease; Viral disease; outbreak; Epidemic keratoconjunctivitis; loop-mediated isothermal amplification; Anterior segment disease
• ISSN: 0146-6615; 1096-9071
• DOI: 10.1002/jmv.22252

Human adenoviruses species D (HAdV‐D) are known to cause severe epidemic keratoconjunctivitis. However, the isolation rate of HAdV‐D is not high, because HAdV‐D is usually slow to propagate. Although new types of HAdV‐D have been reported, accurate surveillance has not been performed because of difficulties in culturing the viruses and lack of a practical identification method. In this study, HAdV‐Ds were detected and identified from patients with epidemic keratoconjunctivitis in the Fukui Prefecture during 1995–2010 by PCR, loop‐mediated isothermal amplification (LAMP) of DNA, and conventional virus isolation and neutralization tests. All samples were subjected to culture and PCR and LAMP. A total of 124 strains of HAdV‐D were detected from 157 patients with epidemic keratoconjunctivitis. The strains consisted of the following types: D8 (n = 8), D19 (n = 4), D37 (n = 40), D53 (n = 5), D54 (n = 66), and D56 (n = 1). Among these, D53, D54, and D56 are new types that have been reported recently. The results of this study demonstrated that new types of HAdV‐D caused epidemic keratoconjunctivitis during 1995–2010, and included an outbreak of keratoconjunctivitis caused by HAdV‐D54. The LAMP method was able to detect and identify HAdV‐D53 and HAdV–D54 in 1 hr, and may therefore be applicable for use at the bedside. J. Med. Virol. 84:81–86, 2011. © 2011 Wiley Periodicals, Inc.