Effect of Organic Enrichment and Desalination on Sample Pretreatment for Non-targeted Analysis of Dissolved Organic Matter in Urban River Water by LC-ESI-Orbitrap MS

High-performance liquid chromatography/high-resolution mass spectrometry (LC/HRMS) can be used to perform non-targeted analysis of dissolved organic matter (DOM) in environmental water at the molecular level without specifying target analytes prior to measurement. Pretreatment of samples subjected t...

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Bibliographic Details
Published inJournal of Water and Environment Technology Vol. 23; no. 3; pp. 155 - 166
Main Authors Kasuga, Ikuro, Furumai, Hiroaki, Kurisu, Futoshi, Uehara, Yutaro
Format Journal Article
LanguageEnglish
Published Tokyo Japan Society on Water Environment 2025
Japan Science and Technology Agency
Subjects
Anions
Chromatography
Desalination
direct injection
Dissolved organic matter
Evaporation
Freeze drying
High performance liquid chromatography
high-resolution mass spectrometry
HPLC
Liquid chromatography
Mass spectrometry
Mass spectroscopy
Pretreatment
River water
Rivers
rotary evaporation
Sewage
Sewage effluents
Solid phases
solid-phase extraction
Water analysis
Water desalting
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Summary:High-performance liquid chromatography/high-resolution mass spectrometry (LC/HRMS) can be used to perform non-targeted analysis of dissolved organic matter (DOM) in environmental water at the molecular level without specifying target analytes prior to measurement. Pretreatment of samples subjected to LC/HRMS strongly affects the comprehensiveness of the analysis. In this study, the use of freeze-drying, vacuum vortex evaporation, and solid-phase extraction (SPE) for sample pretreatment was investigated as well as direct injection of untreated samples. Organic recovery, anion removal, and detected components were evaluated for Japanese urban river water with a high content of sewage effluent. Among the investigated techniques, SPE effectively removed anions and yielded the largest number of detected DOM components. Using the other three methods resulted in higher recoveries of organic matter than SPE but left residual inorganic ions that inhibited detection. Thus, SPE was determined to be the best single method for non-targeted screening analysis. Considering that some components were only detected using pretreatment methods other than SPE, it is recommended that SPE be combined with other methods to improve the comprehensiveness of the analysis.
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ISSN:1348-2165
1348-2165
DOI:10.2965/jwet.24-121