Cytokine concentration in stored canine erythrocyte concentrates

• Published in: Journal of veterinary emergency and critical care (San Antonio, Tex. : 2000) Vol. 24; no. 3; pp. 259 - 263
• Main Authors: Corsi, Raffaella, McMichael, Maureen A, Smith, Stephanie A, O'Brien, Mauria, Herring, Jennifer, Ngwenyama, Thandeka R, Galligan, Alyssa, Beloshapka, Alison N, Deng, Ping, Swanson, Kelly S
• Format: Journal Article
• Language: English
• Published: United States Blackwell Science on behalf of the American College of Veterinary Emergency and Critical Care 01.05.2014; Blackwell Publishing Ltd
• Subjects: Animals; Blood Preservation - veterinary; chemokines; dogs; Dogs - blood; Dogs - physiology; enzyme-linked immunosorbent assay; Enzyme-Linked Immunosorbent Assay - veterinary; erythrocytes; Erythrocytes - physiology; inflammation; interleukin-1beta; Interleukins - physiology; storage lesion; storage time; transfusion; tumor necrosis factor-alpha; Tumor Necrosis Factor-alpha - physiology; dogs; storage lesion; transfusion; inflammation
• ISSN: 1479-3261; 1476-4431
• DOI: 10.1111/vec.12160

OBJECTIVE: To evaluate the effect of leukoreduction (LR) as compared to standard nonleukoreduced (NLR) units on cytokine concentrations in canine erythrocyte concentrates during regular storage time. DESIGN: Randomized, experimental study. SETTING: University teaching hospital. ANIMALS: Ten random‐source research dogs. INTERVENTIONS: One unit of whole blood was collected from each dog and randomized to standard processing (NLR, n = 5) or prestorage LR (n = 5). All units were stored at 4°C. Samples were aseptically collected from each unit weekly for 5 weeks. On day 35, 1 mL of blood was collected from each unit and submitted for aerobic culture. MEASUREMENT AND MAIN RESULTS: An ELISA assay was used to analyze the concentrations of IL‐1β, IL‐8, TNF‐α, and IL‐10. There were no significant effects of either group or storage time for IL‐1β, IL‐10, or TNF‐α. IL‐8 concentration was significantly increased over the storage period in NLR units, and was significantly higher compared to LR units on days 28 and 35. Aerobic culture was negative on all units. CONCLUSIONS: This study demonstrated a marked, storage time‐dependent accumulation of IL‐8 in canine erythrocyte concentrates. Prestorage LR attenuated the accumulation of IL‐8. This chemokine may contribute to the proinflammatory effects of transfusion of stored erythrocyte concentrates.