Fabrication, properties and bioapplications of cellulose/collagen hydrolysate composite films

• Published in: Carbohydrate polymers Vol. 92; no. 2; pp. 1752 - 1760
• Main Authors: Pei, Ying, Yang, Juan, Liu, Pan, Xu, Min, Zhang, Xianzheng, Zhang, Lina
• Format: Journal Article
• Language: English
• Published: Kidlington Elsevier Ltd 15.02.2013; Elsevier
• Subjects: amino acid composition; Animals; Applied sciences; aqueous solutions; Biocompatibility; biocompatible materials; Biocompatible Materials - chemistry; Biocompatible Materials - toxicity; cell adhesion; Cellulose; Cellulose - chemistry; Cellulose and derivatives; Cercopithecus aethiops; collagen; Collagen - chemistry; Collagen hydrolysate; Composite film; composite materials; COS Cells; crosslinking; Exact sciences and technology; films (materials); Fourier transform infrared spectroscopy; hydrolysates; Hydrolysis; Mechanical Phenomena; mechanical properties; Natural polymers; Non-covalent bond; Physicochemistry of polymers; Proteins; scanning electron microscopy; sodium hydroxide; Sodium Hydroxide - chemistry; toxicity testing; urea; Urea - chemistry; Water - chemistry; Composite film; Biocompatibility; Collagen hydrolysate; Non-covalent bond; Cellulose; Biological properties; Mixing; Aglycone; Cellulose hydrate; Mechanical properties; Cytotoxicity; Composition effect; Crosslinking; Experimental study; In vitro; Protein; Humidity effect; Hydrolysate; Linear polymer; Collagen; Crosslinked polymer; Manufacturing; Intramolecular interaction; Growth from solution
• ISSN: 0144-8617; 1879-1344
• DOI: 10.1016/j.carbpol.2012.11.029

► Cellulose/collagen hydrolysate (RC/CH) films were prepared by a ‘green’ way. ► Combined with RC film improved the CH stability in wet condition. ► Crosslinking with genipin improved the mechanical properties of RC/CH films. ► These composite films showed potential application in the biomaterials field. In this work, a series of cellulose/collagen hydrolysate (RC/CH) films were prepared in NaOH/urea aqueous solution via a simple, low-cost and green pathway. To overcome the disadvantages (brittleness, poor water resistance) of CH as biomaterials, CH was combined with regenerated cellulose (RC) film to construct RC/CH composite materials, leading to vast improvement of the water resistance of CH. Crosslinking with genipin further improved the mechanical properties of the RC/CH films in the wet state. Their structure and properties were characterized by elemental analysis, Fourier transform infra-red (FT-IR) spectra, ultraviolet–visible (UV–vis), scanning electron microscopy (SEM), amino acid analysis, tensile testing, cell adhesion and toxicity tests. The mechanical properties and water resistance of the crosslinked RC/CH films were significantly improved, which made collagen hydrolysate as biomaterial could be used at wet state. Moreover, the RC/CH films exhibited good biocompatibility by proliferation of COS7 cells on the surface, supporting cell adhesion and growth.