Genetic diversity of durum wheat as determined by AFLP in fluorescence

• Published in: Biologia plantarum Vol. 54; no. 1; pp. 198 - 200
• Main Authors: Sciacca, F.,Agricultural Research Council, Acireale (Italy). Mediterranean Crops Research Center, Fichera, C.,Agricultural Research Council, Acireale (Italy). Mediterranean Crops Research Center, Di Silvestro, S.,Science and Technology Park of Sicily, Catania (Italy), Conte, E.,Science and Technology Park of Sicily, Catania (Italy), Palumbo, M.,Agricultural Research Council, Acireale (Italy). Mediterranean Crops Research Center
• Format: Journal Article
• Language: English
• Published: Dordrecht Springer Netherlands 01.03.2010; Institute of Experimental Botany of the Czech Academy of Sciences
• Subjects: Biomedical and Life Sciences; BLE; Brief Communication; DNA FINGERPRINTING; EMPREINTE ADN; FILOGENIA; GENETIC POLYMORPHISM; genetic relationship; GENETIC VARIATION; GENOTIPOS; GENOTYPE; genotype identification; GENOTYPES; http://www.fao.org/aos/agrovoc#c_13325; http://www.fao.org/aos/agrovoc#c_15975; http://www.fao.org/aos/agrovoc#c_24031; http://www.fao.org/aos/agrovoc#c_3225; http://www.fao.org/aos/agrovoc#c_34258; http://www.fao.org/aos/agrovoc#c_7955; http://www.fao.org/aos/agrovoc#c_7958; http://www.fao.org/aos/agrovoc#c_8373; HUELLAS GENETICAS ADN; Life Sciences; pattern analysis; PHYLOGENIE; PHYLOGENY; Plant Sciences; POLIMORFISMO GENETICO; POLYMORPHISME GENETIQUE; TRIGO; TRITICUM DURUM; TRITICUM TURGIDUM; triticum turgidum l. ssp. durum; VARIACION GENETICA; VARIATION GENETIQUE; WHEATS; genotype identification; pattern analysis; L. ssp; genetic relationship
• ISSN: 0006-3134; 1573-8264
• DOI: 10.1007/s10535-010-0035-x

The DNA of fifteen Italian cultivars of durum wheat (Triticum turgidum ssp. durum) were analyzed by in fluorescence amplified fragment length polymorphism (fAFLP) in order to obtain the characteristic fingerprintings of genotypes and assess their genetic relatedness. Among 64 combinations of fluorescence labelled primers, three different combinations were chosen as producing a total of 6,630 AFLP fragments, 2,277 (34.3 %) of them being polymorphic. By using this fAFLP methodology a DNA fingerprinting of each durum wheat cultivar was generated for genotype identification. Analysis of the genetic relationships show the low variability among durum wheat cultivars.