Growth interactions between the raphidophyte Chattonella antiqua and the dinoflagellate Akashiwo sanguinea

• Published in: Harmful algae Vol. 11; pp. 81 - 87
• Main Authors: Qiu, Xuchun, Yamasaki, Yasuhiro, Shimasaki, Yohei, Gunjikake, Hiroaki, Matsubara, Tadashi, Nagasoe, Sou, Etoh, Takuya, Matsui, Shigeaki, Honjo, Tsuneo, Oshima, Yuji
• Format: Journal Article
• Language: English
• Published: Elsevier B.V 01.11.2011
• Subjects: Akashiwo sanguinea; Allelopathy; axenic culture; Cell contact; cell culture; Chattonella antiqua; density; Dinophyceae; eukaryotic cells; Growth interaction; Growth simulation; interspecific competition; mathematical models; microbial growth; Allelopathy; Growth interaction; Akashiwo sanguinea; Chattonella antiqua; Cell contact; Growth simulation
• ISSN: 1568-9883; 1878-1470
• DOI: 10.1016/j.hal.2011.08.001

► We investigated the growth interactions between C. antiqua and A. sanguinea. ► One of the two species always inhibits the growth of the other. ► Relative initial cell densities are critical in determining the competition winner. ► Both allelopathy and cell contact are involved in growth interactions between them. We investigated the growth interactions between the raphidophyte Chattonella antiqua and the dinoflagellate Akashiwo sanguinea by using bi-algal cultures under axenic conditions. When initial cell densities of C. antiqua and A. sanguinea both were 1 × 10 2 cells ml −1, C. antiqua grew faster and its growth inhibitory effect on A. sanguinea was stronger than that of A. sanguinea on C. antiqua. In other combinations of inoculation densities, the species that was inoculated at lower cell density (1 × 10 2 cells ml −1) stopped growing soon after the start of the experiments, and cell density of this species gradually decreased after the other species reached early stationary phase. The mathematical model used to simulate the growth and interactions between the two species predicts that C. antiqua and A. sanguinea will approach an unstable equilibrium point of about 2.16 × 10 4 cells ml −1 ( C. antiqua) and 1.46 × 10 4 cells ml −1 ( A. sanguinea). Thus, one of the species always inhibits the growth of the other and their initial cell densities are critical in determining the successful species in growth competition. When one of these two species was inoculated at the cell density of 2 × 10 3 cells ml −1 in bi-algal culture experiments under noncontact and contact conditions, its growth inhibitory effect toward the other species was greater under contact conditions than under noncontact conditions. Furthermore, enriched filtrate from dense culture of C. antiqua resulted in both reduced maximum yield and maximum growth rate of A. sanguinea, whereas that of A. sanguinea resulted only in reduced maximum yield of C. antiqua but did not affect its growth rate.