Gas chromatographic separation of the enantiomers of bromocyclen in fish samples

• Published in: Chemosphere (Oxford) Vol. 29; no. 7; pp. 1385 - 1391
• Main Authors: Pfaffenberger, Bernd, Hühnerfuss, Heinrich, Gehrcke, Bärbel, Hardt, Ingo, König, Wilfried A., Rimkus, Gerhard
• Format: Journal Article
• Language: English
• Published: Oxford Elsevier Ltd 1994; Elsevier
• Subjects: ambramis brama orientalis; Animal, plant and microbial ecology; Applied ecology; Biological and medical sciences; bromocyclen; Cyprinidae; Ecotoxicology, biological effects of pollution; enantiomers; Fundamental and applied biological sciences. Psychology; gas chromatography; insecticide residues; leuciscus idus; muscles; Oncorhynchus mykiss; pike; Techniques; Denmark; Germany; Gas chromatography; Tissue; Vertebrata; Insecticide; Separation; Pisces; Enantiomer; Parasiticid; Oncorhynchus mykiss
• ISSN: 0045-6535; 1879-1298
• DOI: 10.1016/0045-6535(94)90272-0

In muscle tissue of rainbow trouts from Danish fish farms and of three different fish species (orfe, bream, and pike) from the river Stör (northern Germany) remarkably high concentrations (between 0.09 and 1.23 mg/kg fat) of the chiral insecticide bromocyclen were found. For the first time, the separation of the bromocyclen enantiomers in environmental samples was achieved with the help of chiral capillary gas chromatography using modified cyclodextrin phases. A preferential enzymatic metabolism of the first eluting (−)-enantiomer was verified. The toxic implication of this result as well as the sources for the contamination are not yet known.