Epitope-defined Monoclonal Antibodies against Multiplexin Collagens Demonstrate that Type XV and XVIII Collagens are Expressed in Specialized Basement Membranes

• Published in: Cell Structure and Function Vol. 27; no. 1; pp. 9 - 20
• Main Authors: Tomono, Yasuko, Naito, Ichiro, Ando, Kaori, Yonezawa, Tomoko, Sado, Yoshikazu, Hirakawa, Satoshi, Arata, Jirô, Okigaki, Tohru, Ninomiya, Yoshifumi
• Format: Journal Article
• Language: English
• Published: Japan Japan Society for Cell Biology 01.02.2002; Japan Science and Technology Agency
• Subjects: Antibodies, Monoclonal - immunology; basement membrane; Basement Membrane - metabolism; Capillaries - metabolism; Capillaries - ultrastructure; capillary; Collagen - immunology; Collagen - metabolism; Collagen Type XVIII; Epithelial Cells - metabolism; Epithelial Cells - ultrastructure; Epitopes - immunology; Humans; Immunohistochemistry; Muscles - metabolism; Muscles - ultrastructure; smooth muscle cell; Tissue Distribution; type XV collagen; type XVIII collagen
• ISSN: 0386-7196; 1347-3700
• DOI: 10.1247/csf.27.9

Type XV and type XVIII collagens are classified as part of multiplexin collagen superfamily and their C-terminal parts, endostatin and restin, respectively, have been shown to be anti-angiogenic in vivo and in vitro. The α1(XV) and α1(XVIII) collagen chains are reported to be localized mainly in the basement membrane zone, but their distributions in blood vessels and nonvascular tissues have yet to be thoroughly clarified. In the present study, we raised monoclonal antibodies against synthetic peptides of human α1(XV) and α1(XVIII) chains and used them for extensive investigation of the distribution of these chains. We came to the conclusion that nonvascular BMs contain mainly one of two types: subepithelial basement membranes that contained type XVIII in general, or skeletal and cardiac muscles that harbored mainly type XV. But basement membranes surrounding smooth muscle cells in vascular tissues contained one or both of them, depending on their locations. Interestingly, continuous capillaries contained both type XV and type XVIII collagens in their basement membranes; however, fenestrated or specialized capillaries such as glomeruli, liver sinusoids, lung alveoli, and splenic sinusoids expressed only type XVIII in their basement membranes, lacking type XV. This observation could imply that different functions of basement membranes in various tissues and organs use different mechanisms for the endogenous control of angiogenesis.