Endophytic fungi of Brunfelsia uniflora: isolation, cryopreservation, and determination of enzymatic and antioxidant activity
Brunfelsia uniflora (Pohl.) D. Don (Solanaceae), commonly known as manacá-de-cheiro , is widely distributed in Brazil and used by local indigenous peoples as an antirheumatic, antisyphilitic, depurative, emetic, vermifuge, and purgative agent. Several studies have examined the biological activities...
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Published in | World journal of microbiology & biotechnology Vol. 38; no. 6; p. 94 |
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Main Authors | , , , , , , , , , , |
Format | Journal Article |
Language | English |
Published |
Dordrecht
Springer Netherlands
01.06.2022
Springer Nature B.V |
Subjects | |
Online Access | Get full text |
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Summary: | Brunfelsia uniflora
(Pohl.) D. Don (Solanaceae), commonly known as
manacá-de-cheiro
, is widely distributed in Brazil and used by local indigenous peoples as an antirheumatic, antisyphilitic, depurative, emetic, vermifuge, and purgative agent. Several studies have examined the biological activities and phytochemical profile of
Brunfelsia
; however, few have focused on the diversity of endophytic microorganisms that colonize members of the genus. This study aimed to isolate and cryopreserve endophytic fungi from
B. uniflora
and determine their cellulase, laccase, and antioxidant activities. Endophytic fungi were isolated from
B. uniflora
stems, cultured on wheat grains, immersed in a 150 g L
−1
aqueous sucrose solution, and cryopreserved at – 80 °C for 1 and 6 months. Cellulase activity was determined by a qualitative test using carboxymethylcellulose medium and laccase activity by a quantitative test based on the oxidation of 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonate). Prior to antioxidant activity assays, fungi were grown in malt extract broth for production of mycelial biomass. A methanolic extract was prepared for evaluation of
DPPH
·
scavenging activity, FRAP activity, and total phenolic content. A total of 46 endophytic fungal isolates were obtained from
B. uniflora
stems and classified into 24 groups according to morphological similarities.
B. uniflora
was shown to harbor different genera of ascomycete fungi as endophytic organisms
.
Mycelial viability was observed after 1 and 6 months of cryopreservation at − 80 °C. Fungi exhibited cellulase and laccase activities. Isolate CE23 had the highest laccase activity after 7 days of cultivation. Twelve isolates were found to have low total phenolic contents and
DPPH
·
and FRAP activities. |
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Bibliography: | ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-3 content type line 23 ObjectType-Review-1 |
ISSN: | 0959-3993 1573-0972 |
DOI: | 10.1007/s11274-022-03278-5 |