Enzyme PTP-1B Inhibition Studies by Vanadium Metal Complexes: a Kinetic Approach

• Published in: Biological trace element research Vol. 201; no. 10; pp. 5037 - 5052
• Main Authors: Shaik, Ayub, Kondaparthy, Vani, Begum, Alia, Husain, Ameena, Manwal, Deva Das
• Format: Journal Article
• Language: English
• Published: New York Springer US 01.10.2023; Springer Nature B.V
• Subjects: Analytical methods; Biochemistry; Biomedical and Life Sciences; Biotechnology; Coordination compounds; Diabetes; Diabetes mellitus; Emission spectroscopy; Enzyme kinetics; Enzymes; Fluorescence; Hormones; Inhibitors; Insulin; Kinetics; Leptin; Life Sciences; Metal complexes; Metals; Molecular modelling; Nutrition; Obesity; Oncology; Proteins; Spectroscopy; Vanadium; Serum glucose; PTP-1B; IC; Vanadium complexes; Kinetic studies; V max; IC50; K m
• ISSN: 0163-4984; 1559-0720
• DOI: 10.1007/s12011-023-03557-8

The medical field now needs more novel drugs to treat obesity and type-2 diabetes mellitus (T2D) than ever before. Obesity and T2D are both characterized by resistance to the hormones leptin and insulin. PTP-1B is a promising target for drug growth, as strong genetic, pharmacological, and biochemical evidence points to the possibility of treating diabetes and obesity by blocking the PTP-1B enzyme. Studies have also found that PTP-1B is overexpressed in patients with diabetes and obesity, suggesting that inhibiting PTP-1B may be a useful technique in their care. There are no clinically used PTP-1B inhibitors, despite the fact that numerous naturally occurring PTP-1B inhibitors have demonstrated great therapeutic promise. This is most likely due to their low activity or lack of selectivity. It is still important to look for more effective and focused PTP-1B inhibitors. A few organovanadium metal complexes were synthesized and characterized, and binding studies on vanadium complexes with PTP-B were also performed using fluorescence emission spectroscopy. Additionally, we theoretically (molecular modeling) and experimentally (enzyme kinetics) examined the PTP-1B inhibitory effects of these vanadium metal complexes and found that they have excellent PTP-1B inhibitory properties.