On Interactions of Sulfamerazine with Cyclodextrins from Coupled Diffusometry and Toxicity Tests

• Published in: Biomolecules (Basel, Switzerland) Vol. 14; no. 4; p. 462
• Main Authors: Sofio, Sara P C, Caeiro, André, Ribeiro, Ana C F, Cabral, Ana M T D P V, Valente, Artur J M, Canhoto, Jorge, Esteso, Miguel A
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 01.04.2024
• Subjects: 2-Hydroxypropyl-beta-cyclodextrin - chemistry; antibiotics; Antimicrobial agents; Aqueous solutions; beta-Cyclodextrins - chemistry; Cyclodextrin; Cyclodextrins; Cyclodextrins - chemistry; Diffusion; Drugs; Evaluation; Germination; Glucose; Methods; Molecular biology; Pharmaceuticals; Physiological aspects; Pollen; Statistical analysis; Sulfamerazine; Sulfamerazine - chemistry; Sulfamethazine; Toxicity; Toxicity testing; Toxicity Tests; transport properties; Variance analysis; Portugal; United States > US; Netherlands; Germany; diffusion; toxicity tests; sulfamerazine; antibiotics; cyclodextrins; transport properties
• ISSN: 2218-273X; 2218-273X
• DOI: 10.3390/biom14040462

This scientific study employs the Taylor dispersion technique for diffusion measurements to investigate the interaction between sulfamerazine (NaSMR) and macromolecular cyclodextrins ( -CD and HP- -CD). The results reveal that the presence of -CD influences the diffusion of the solution component, NaSMR, indicating a counterflow of this drug due to solute interaction. However, diffusion data indicate no inclusion of NaSMR within the sterically hindered HP- -CD cavity. Additionally, toxicity tests were conducted, including pollen germination ( ) and growth curve assays in BY-2 cells. The pollen germination tests demonstrate a reduction in sulfamerazine toxicity, suggesting potential applications for this antimicrobial agent with diminished adverse effects. This comprehensive investigation contributes to a deeper understanding of sulfamerazine-cyclodextrin interactions and their implications for pharmaceutical and biological systems.