Electrochemical biosensor based on self-assembled monolayers modified with gold nanoparticles for detection of HER-3

• Published in: Analytica chimica acta Vol. 814; pp. 31 - 38
• Main Authors: Canbaz, Mehmet Çetin, Şimşek, Çiğdem Sayıklı, Sezgintürk, Mustafa Kemal
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 03.03.2014
• Subjects: Anti-HER-3; Antibodies, Immobilized - chemistry; Biosensing Techniques - methods; Biosensor; Biosensors; Cancer biomarkers; Cysteamine; Cysteamine - chemistry; Dielectric Spectroscopy - methods; Electrochemical impedance spectroscopy; Electrodes; Gold; Gold - chemistry; HER-3; Humans; Impedance; Limit of Detection; Mathematical analysis; Nanoparticles; Nanoparticles - chemistry; Receptor, ErbB-3 - analysis; Single frequency impedance; Cancer biomarkers; Single frequency impedance; HER-3; Anti-HER-3; Biosensor
• ISSN: 0003-2670; 1873-4324
• DOI: 10.1016/j.aca.2014.01.041

•Single frequency impedance technique was firstly used for characterization of interaction between HER-3 and anti-HER-3.•Anti-HER-3 antibody was firstly utilized in an immunosensor as a bioreceptor.•The biosensor exhibits high analytical performance with a linear range 0.2–1.4pgmL−1.•Kramers–Kronig transform was successfully performed on the experimental impedance data. We have developed a new immunological biosensor for ultrasensitive quantification of human epidermal growth factor receptor-3(HER-3). In order to construct the biosensor, the gold electrode surface was layered with, hexanedithiol, gold nanoparticles, and cysteamine, respectively. Anti-HER-3 antibody was covalently attached to cysteamine by glutaraldehyde and used as a bioreceptor in a biosensor system for the first time by this study. Surface characterization was obtained by means of electrochemical impedance spectroscopy and voltammetry. The proposed biosensor showed a good analytical performance for the detection of HER-3 ranging from 0.2 to 1.4pgmL−1. Kramers–Kronig transform was performed on the experimental impedance data. Moreover, in an immunosensor system, the single frequency impedance technique was firstly used for characterization of interaction between HER-3 and anti-HER-3. Finally the presented biosensor was applied to artificial serum samples spiked with HER-3.