trypanosome alternative oxidase exists as a monomer in Trypanosoma brucei mitochondria

• Published in: Parasitology research (1987) Vol. 96; no. 3; pp. 178 - 183
• Main Authors: CHAUDHURI, Minu, OTT, Robert Daniel, SAHA, Lipi, WILLIAMS, Shuntae, HILL, George C
• Format: Journal Article
• Language: English
• Published: Berlin Springer-Verlag 01.06.2005; Springer
• Subjects: Animals; Biological and medical sciences; Dimerization; Electrophoresis, Polyacrylamide Gel; Fundamental and applied biological sciences. Psychology; General aspects; General aspects and techniques. Study of several systematic groups. Models; Invertebrates; Mitochondria - enzymology; Mitochondrial Proteins; Oxidoreductases - chemistry; Oxidoreductases - metabolism; Plant Proteins; Protozoan Proteins - chemistry; Protozoan Proteins - metabolism; Reducing Agents; Trypanosoma brucei brucei - enzymology; Kinetoplastida; Protozoa; Mitochondria; Enzyme; Oxidase; Parasite; Oxidoreductases; Trypanosoma brucei
• ISSN: 0932-0113; 1432-1955
• DOI: 10.1007/s00436-005-1337-3

The bloodstream forms of African trypanosomes solely depend on trypanosome alternative oxidase (TAO), for respiration. Similar to alternative oxidases (AOXs) found in plants and fungi, TAO is a membrane-bound diiron protein. Here, we investigated if TAO exists as a dimer like plant AOXs, or as a monomer like that of fungi. We have found that TAO forms a homo-dimer on a regular SDS-PAGE in the absence of any reducing agent and exists as a monomer under reducing condition. However, TAO does not form a dimer upon treatment of mitochondria with diamide. TAO was found as a higher molecular mass complex on a Blue-native gel after solubilization with digitonin. In the detergent soluble form, TAO activity was stimulated under reducing and inhibited under oxidizing condition. However, these conditions have no effect on the TAO activity in the mitochondria. Moreover, chemical cross-linking analysis revealed that TAO could not be cross-linked when present in the mitochondria. Together, it suggests that like certain other hydrophobic membrane proteins, TAO forms a dimer or oligomer when solubilized with detergents, and the TAO-dimer is SDS-resistant. However, it exists as a monomer in Trypanosoma brucei mitochondria.